Identification of a conserved glycan signature for microvesicles

Bianca S. Batista, William S. Eng, Kanoelani T. Pilobello, Karen D. Hendricks-Muñoz, Lara Mahal

Research output: Contribution to journalArticle

Abstract

Microvesicles (exosomes) are important mediators of intercellular communication, playing a role in immune regulation, cancer progression, and the spread of infectious agents. The biological functions of these small vesicles are dependent on their composition, which is regulated by mechanisms that are not well understood. Although numerous proteomic studies of these particles exist, little is known about their glycosylation. Carbohydrates are involved in protein trafficking and cellular recognition. Glycomic analysis may thus provide valuable insights into microvesicle biology. In this study, we analyzed glycosylation patterns of microvesicles derived from a variety of biological sources using lectin microarray technology. Comparison of the microvesicle glycomes with their parent cell membranes revealed both enrichment and depletion of specific glycan epitopes in these particles. These include enrichment in high mannose, polylactosamine, α-2,6 sialic acid, and complex N-linked glycans and exclusion of terminal blood group A and B antigens. The polylactosamine signature derives from distinct glycoprotein cohorts in microvesicles of different origins. Taken together, our data point to the emergence of microvesicles from a specific membrane microdomain, implying a role for glycosylation in microvesicle protein sorting.

Original languageEnglish (US)
Pages (from-to)4624-4633
Number of pages10
JournalJournal of Proteome Research
Volume10
Issue number10
DOIs
StatePublished - Oct 7 2011

Fingerprint

Glycosylation
Polysaccharides
Protein Transport
Glycomics
Membrane Microdomains
Exosomes
N-Acetylneuraminic Acid
Cell membranes
Microarrays
Mannose
Blood Group Antigens
Sorting
Lectins
Proteomics
Epitopes
Glycoproteins
Proteins
Carbohydrates
Cell Membrane
Technology

Keywords

  • carbohydrates
  • exosomes
  • glycan
  • glycomics
  • lectin microarray
  • microvesicles

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Batista, B. S., Eng, W. S., Pilobello, K. T., Hendricks-Muñoz, K. D., & Mahal, L. (2011). Identification of a conserved glycan signature for microvesicles. Journal of Proteome Research, 10(10), 4624-4633. https://doi.org/10.1021/pr200434y

Identification of a conserved glycan signature for microvesicles. / Batista, Bianca S.; Eng, William S.; Pilobello, Kanoelani T.; Hendricks-Muñoz, Karen D.; Mahal, Lara.

In: Journal of Proteome Research, Vol. 10, No. 10, 07.10.2011, p. 4624-4633.

Research output: Contribution to journalArticle

Batista, BS, Eng, WS, Pilobello, KT, Hendricks-Muñoz, KD & Mahal, L 2011, 'Identification of a conserved glycan signature for microvesicles', Journal of Proteome Research, vol. 10, no. 10, pp. 4624-4633. https://doi.org/10.1021/pr200434y
Batista, Bianca S. ; Eng, William S. ; Pilobello, Kanoelani T. ; Hendricks-Muñoz, Karen D. ; Mahal, Lara. / Identification of a conserved glycan signature for microvesicles. In: Journal of Proteome Research. 2011 ; Vol. 10, No. 10. pp. 4624-4633.
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