Identification of a candidate primary sex determination locus, fox-1, on the X chromosome of Caenorhabditis elegans

J. Hodgkin, J. D. Zellan, D. G. Albertson

Research output: Contribution to journalArticle

Abstract

Sex in Caenorhabditis elegans (XX hermaphrodite, XO male) is determined by the X:A ratio, which is the ratio of X chromosome number to autosomal set number. Recent genetic results with X chromosome duplications have suggested that there may be only a small number of major numerator sites on the X chromosome that contribute to this ratio. Mapping of duplication endpoints delimited a region of less than 300 kb, likely to contain one such element. Cosmid clones from this region were tested for numerator activity by constructing transgenic lines carrying extra copies of each tested cosmid. Most cosmid arrays have no effect on the viability of either XX or XO animals. One cosmid array was found to be viable in XX animals, but lethal and feminizing in XO animals, consistent with it containing a major numerator element. Further experiments defined a region of 12-30 kb with apparent numerator activity, which is designated fox-1, 'Feminizing locus on X'. A cDNA clone hybridizing across part of this region encodes a predicted RNA-binding protein.

Original languageEnglish (US)
Pages (from-to)3681-3689
Number of pages9
JournalDevelopment
Volume120
Issue number12
StatePublished - Jan 1 1994

    Fingerprint

Keywords

  • Caenorhabditis elegans
  • Nematode
  • Numerator elements
  • Sex determination
  • X:A ratio

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this