Identification and structural characterization of an unusual RING-like sequence within an extracellular biomineralization protein, AP7

Sebastiano Collino, Il Kim Kim, John Evans

Research output: Contribution to journalArticle

Abstract

The RING or Really Interesting New Gene represents a family of eukaryotic sequences that bind Zn (II) ions and participate in intracellular processes involving protein-protein interaction. Although found in over 400 different proteins, very little is known regarding the structure-function properties of these domains because of the aggregation problems associated with RING sequences. To augment this data set, we report an unusual 36 AA C-terminal sequence of an extracellular matrix mollusk shell protein, AP7, that exhibits partial homology to the RING family. This Cys, His-containing sequence, termed AP7C, binds Zn (II) and other multivalent ions, but does not utilize a tetracoordinate complexation scheme for binding such as that found in Zn (II) finger polypeptides. Moreover, unlike Zn (II) finger and RING domains, this 36 AA can fold into a relatively stable structure in the absence of Zn (II). This folded structure consists of three short helical segments (A, B, and C), with segments A and B separated by a 4 AA type I β-turn region and segments B and C separated by a 7 AA loop-like region. Interestingly, the putative RING-like region, -RRPFHECALCYSI-, experiences slow conformational exchange between two structural states in solution, most likely in response to imido ring interconversion at P8 and P21. Poisson-Boltzmann solvation calculations reveal that the AP7C molecular surface possesses a cationic region near its N-terminus, which lies adjacent to the 30 AA mineral modification domain in the AP7 protein. Given that the AP7C sequence does not influence mineralization, it is probable that this cationic pseudo-RING region is utilized by the AP7 protein for other tasks such as protein-protein interaction within the mollusk shell matrix.

Original languageEnglish (US)
Pages (from-to)3745-3755
Number of pages11
JournalBiochemistry
Volume47
Issue number12
DOIs
StatePublished - Mar 25 2008

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Biomineralization
Proteins
Mollusca
RING Finger Domains
Ions
Solvation
Complexation
Fingers
Extracellular Matrix
Minerals
Ion exchange
Agglomeration
Genes
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification and structural characterization of an unusual RING-like sequence within an extracellular biomineralization protein, AP7. / Collino, Sebastiano; Kim, Il Kim; Evans, John.

In: Biochemistry, Vol. 47, No. 12, 25.03.2008, p. 3745-3755.

Research output: Contribution to journalArticle

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