Identification and characterization of a short 2′-3′ bond-forming ribozyme

Duncan Smith, Maria M. Konarska

Research output: Contribution to journalArticle

Abstract

A large number of natural and artificial ribozymes have been isolated since the demonstration of the catalytic potential of RNA, with the majority of these catalyzing phosphate hydrolysis or transesterification reactions. Here, we describe and characterize an extremely short ribozyme that catalyzes the positionally specific transesterification that produces a 2′-3′ phosphodiester bond between itself and a branch substrate provided in trans, cleaving itself internally in the process. Although this ribozyme was originally derived from constructs based on snRNAs, its minimal catalytic motif contains essentially no snRNA sequence and the reaction it catalyzes is not directly related to either step of pre-mRNA splicing. Our data have implications for the intrinsic reactivity of the large amount of RNA sequence space known to be transcribed in nature and for the validity and utility of the use of protein-free systems to study pre-mRNA splicing. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)8-13
Number of pages6
JournalRNA
Volume15
Issue number1
DOIs
StatePublished - Jan 2009

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Catalytic RNA
RNA Precursors
Small Nuclear RNA
Hydrolysis
Phosphates
Proteins

Keywords

  • RNA catalysis
  • snRNAs
  • Splicing

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Identification and characterization of a short 2′-3′ bond-forming ribozyme. / Smith, Duncan; Konarska, Maria M.

In: RNA, Vol. 15, No. 1, 01.2009, p. 8-13.

Research output: Contribution to journalArticle

Smith, Duncan ; Konarska, Maria M. / Identification and characterization of a short 2′-3′ bond-forming ribozyme. In: RNA. 2009 ; Vol. 15, No. 1. pp. 8-13.
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