Hyaluronan and Hyaluronan Fragments

Research output: Contribution to journalArticle

Abstract

The glycosaminoglycan hyaluronan (HA) is a key component of the microenvironment surrounding cells. In healthy tissues, HA molecules have extremely high molecular mass and consequently large hydrodynamic volumes. Tethered to the cell surface by clustered receptor proteins, HA molecules crowd each other, as well as other macromolecular species. This leads to severe nonideality in physical properties of the biomatrix, because steric exclusion leads to an increase in effective concentration of the macromolecules. The excluded volume depends on both polymer concentration and hydrodynamic volume/molecular mass. The biomechanical properties of the extracellular matrix, tissue hydration, receptor clustering, and receptor-ligand interactions are strongly affected by the presence of HA and by its molecular mass. In inflammation, reactive oxygen and nitrogen species fragment the HA chains. Depending on the rate of chain degradation relative to the rates of new synthesis and removal of damaged chains, short fragments of the HA molecules can be present at significant levels. Not only are the physical properties of the extracellular matrix affected, but the HA fragments decluster their primary receptors and act as endogenous danger signals. Bioanalytical methods to isolate and quantify HA fragments have been developed to determine profiles of HA content and size in healthy and diseased biological fluids and tissues. These methods have potential use in medical diagnostic tests. Therapeutic agents that modulate signaling by HA fragments show promise in wound healing and tissue repair without fibrosis.

Original languageEnglish (US)
JournalAdvances in Carbohydrate Chemistry and Biochemistry
DOIs
StateAccepted/In press - Jan 1 2017

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Hyaluronic Acid
Molecular mass
Tissue
Hydrodynamics
Molecules
Extracellular Matrix
Physical properties
Cellular Microenvironment
Reactive Nitrogen Species
Cell Surface Receptors
Glycosaminoglycans
Macromolecules
Routine Diagnostic Tests
Hydration
Wound Healing
Cluster Analysis
Reactive Oxygen Species
Polymers
Fibrosis
Repair

Keywords

  • Biotherapeutics
  • Cell receptors
  • Cell signaling
  • Extracellular matrix
  • Extracellular vesicles
  • Free radicals
  • Glycosaminoglycan
  • Hyaluronan
  • Inflammation
  • Macromolecular crowding
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

Cite this

Hyaluronan and Hyaluronan Fragments. / Cowman, Mary.

In: Advances in Carbohydrate Chemistry and Biochemistry, 01.01.2017.

Research output: Contribution to journalArticle

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