Humans immunized with Streptococcus mutans antigens by mucosal routes

N. K. Childers, G. Tong, F. Li, Ananda Dasanayake, K. Kirk, S. M. Michalek

Research output: Contribution to journalArticle

Abstract

Strategies aimed at the prevention of Streptococcus mutans infection and dental caries include mucosal immunization, which results in salivary anti-S. mutans responses. The purpose of this study was to evaluate the effectiveness of nasal vs. tonsillar immunization with S. mutans antigens in inducing salivary immune responses. Twenty-one adult subjects were immunized twice, within a seven-day interval, with a glucosyltransferase-enriched preparation (E-GTF) administered by nasal or tonsillar topical spray. Parotid saliva, nasal wash, and serum were collected prior to and at one- to two-week intervals for 3 months following immunization and were assayed by ELISA for anti-E-GTF activity. Results were analyzed by means of the mixed-models procedure with p < 0.05 level of significance. Significantly higher anti-E-GTF responses were detected in saliva and nasal wash samples from the group immunized by the nasal compared with the tonsillar route, indicating that nasal immunization was more effective in inducing mucosal responses in adults.

Original languageEnglish (US)
Pages (from-to)48-52
Number of pages5
JournalJournal of Dental Research
Volume81
Issue number1
StatePublished - 2002

Fingerprint

Streptococcus mutans
Nose
Antigens
Immunization
Saliva
Glucosyltransferases
Dental Caries
Enzyme-Linked Immunosorbent Assay
Infection
Serum

Keywords

  • Caries
  • Immunization
  • Liposomes
  • Mucosal immunity
  • Streptococcus mutans

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Childers, N. K., Tong, G., Li, F., Dasanayake, A., Kirk, K., & Michalek, S. M. (2002). Humans immunized with Streptococcus mutans antigens by mucosal routes. Journal of Dental Research, 81(1), 48-52.

Humans immunized with Streptococcus mutans antigens by mucosal routes. / Childers, N. K.; Tong, G.; Li, F.; Dasanayake, Ananda; Kirk, K.; Michalek, S. M.

In: Journal of Dental Research, Vol. 81, No. 1, 2002, p. 48-52.

Research output: Contribution to journalArticle

Childers, NK, Tong, G, Li, F, Dasanayake, A, Kirk, K & Michalek, SM 2002, 'Humans immunized with Streptococcus mutans antigens by mucosal routes', Journal of Dental Research, vol. 81, no. 1, pp. 48-52.
Childers, N. K. ; Tong, G. ; Li, F. ; Dasanayake, Ananda ; Kirk, K. ; Michalek, S. M. / Humans immunized with Streptococcus mutans antigens by mucosal routes. In: Journal of Dental Research. 2002 ; Vol. 81, No. 1. pp. 48-52.
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