Human satellite progenitor cells for use in myofascial repair

Isolation and characterization

Melissa S. Logan, John T. Propst, James M. Nottingham, Richard L. Goodwin, Diego F. Pabon, Louis Terracio, Michael J. Yost, Stephen A. Fann

Research output: Contribution to journalArticle

Abstract

Current use of prosthetic meshes and implants for myofascial reconstruction has been associated with infectious complications, long-term failure, and dissatisfying cosmetic results. Our laboratory has developed a small animal model for ventral hernia repair, which uses progenitor cells isolated from a skeletal muscle biopsy. In the model, progenitor cells are expanded in vitro, seeded onto a nonimmunogenic, novel aligned scaffold of bovine collagen and placed into the defect as a living adjuvant to the innate repair mechanism. The purpose of the current investigation is to examine the feasibility of translating our current model to humans. As a necessary first step we present our study on the efficacy of isolating satellite cells from 9 human donor biopsies. We were able to successfully translate our progenitor cell isolation and culture protocols to a human model with some modifications. Specifically, we have isolated human satellite muscle cells, expanded them in culture, and manipulated these cells to differentiate into myotubes in vitro. Immunohistochemical analysis allowed the characterization of distinct progenitor cell cycle stages and quantification of approximate cell number. Furthermore, isolated cells were tracked via cytoplasmic nanocrystal labeling and observed using confocal microscopy.

Original languageEnglish (US)
Pages (from-to)794-799
Number of pages6
JournalAnnals of Plastic Surgery
Volume64
Issue number6
DOIs
StatePublished - Jun 2010

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Stem Cells
Cell Culture Techniques
Ventral Hernia
Biopsy
Cell Separation
Herniorrhaphy
Skeletal Muscle Fibers
Confocal Microscopy
Cosmetics
Nanoparticles
Muscle Cells
Cell Cycle
Skeletal Muscle
Collagen
Animal Models
Cell Count
In Vitro Techniques

ASJC Scopus subject areas

  • Surgery

Cite this

Logan, M. S., Propst, J. T., Nottingham, J. M., Goodwin, R. L., Pabon, D. F., Terracio, L., ... Fann, S. A. (2010). Human satellite progenitor cells for use in myofascial repair: Isolation and characterization. Annals of Plastic Surgery, 64(6), 794-799. https://doi.org/10.1097/SAP.0b013e3181b025cb

Human satellite progenitor cells for use in myofascial repair : Isolation and characterization. / Logan, Melissa S.; Propst, John T.; Nottingham, James M.; Goodwin, Richard L.; Pabon, Diego F.; Terracio, Louis; Yost, Michael J.; Fann, Stephen A.

In: Annals of Plastic Surgery, Vol. 64, No. 6, 06.2010, p. 794-799.

Research output: Contribution to journalArticle

Logan, MS, Propst, JT, Nottingham, JM, Goodwin, RL, Pabon, DF, Terracio, L, Yost, MJ & Fann, SA 2010, 'Human satellite progenitor cells for use in myofascial repair: Isolation and characterization', Annals of Plastic Surgery, vol. 64, no. 6, pp. 794-799. https://doi.org/10.1097/SAP.0b013e3181b025cb
Logan, Melissa S. ; Propst, John T. ; Nottingham, James M. ; Goodwin, Richard L. ; Pabon, Diego F. ; Terracio, Louis ; Yost, Michael J. ; Fann, Stephen A. / Human satellite progenitor cells for use in myofascial repair : Isolation and characterization. In: Annals of Plastic Surgery. 2010 ; Vol. 64, No. 6. pp. 794-799.
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