Human platelet myosin. II. In vitro assembly and structure of myosin filaments

Richard Niederman, T. D. Pollard

Research output: Contribution to journalArticle

Abstract

The authors have used electron microscopy and solubility measurements to investigate the assembly and structure of purified human platelet myosin and myosin rod into filaments. In buffers with ionic strengths of less than 0.3 M, platelet myosin forms filaments which are remarkable for their small size, being only 320 nm long and 10-11 nm wide in the center of the bare zone. The dimensions of these filaments are not affected greatly by variation of the pH between 7 and 8, variation of the ionic strength between 0.05 and 0.2 M, the presence or absence of 1 mM Mg++ or ATP, or variation of the myosin concentration between 0.05 and 0.7 mg/ml. In 1 mM Ca++ and at pH 6.5 the filaments grow slightly larger. More than 90% of purified platelet myosin molecules assemble into filaments in 0.1 M KCl at pH 7. Purified preparations of the tail fragment of platelet myosin also form filaments. These filaments are slightly larger than myosin filaments formed under the same conditions, indicating that the size of the myosin filaments may be influenced by some interaction between the head and tail portions of myosin molecules. Calculations based on the size and shape of the myosin filaments, the dimensions of the myosin molecule and analysis of the bare zone reveal that the synthetic platelet myosin filaments consists of 28 myosin molecules arranged in a bipolar array with the heads of two myosin molecules projecting from the backbone of the filament at 14-15 nm intervals. The heads appear to be loosely attached to the backbone by a flexible portion of the myosin tail. Given the concentration of myosin in platelets and the number of myosin molecules per filament, very few of these thin myosin filaments should be present in a thin section of a platelet, even if all of the myosin molecular are aggregated into filaments.

Original languageEnglish (US)
Pages (from-to)72-92
Number of pages21
JournalJournal of Cell Biology
Volume67
Issue number1
StatePublished - 1975

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Myosins
Blood Platelets
In Vitro Techniques
Osmolar Concentration
Tail
Myosin Subfragments
Platelet Count

ASJC Scopus subject areas

  • Cell Biology

Cite this

Human platelet myosin. II. In vitro assembly and structure of myosin filaments. / Niederman, Richard; Pollard, T. D.

In: Journal of Cell Biology, Vol. 67, No. 1, 1975, p. 72-92.

Research output: Contribution to journalArticle

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