Human platelet myosin. I. Purification by a rapid method applicable to other nonmuscle cells

Thomas D. Pollard, Susan M. Thomas, Richard Niederman

Research output: Contribution to journalArticle

Abstract

Using refinements of established procedures and a new two buffer gel filtration method, it is possible to prepare human platelet myosin which by gel electrophoresis is free from actin and over 95% pure. This method with minor modifications is useful for the purification of myosin from several other nonmuscle cells.

Original languageEnglish (US)
Pages (from-to)258-266
Number of pages9
JournalAnalytical Biochemistry
Volume60
Issue number1
DOIs
StatePublished - 1974

Fingerprint

Myosins
Platelets
Purification
Blood Platelets
Gels
Electrophoresis
Actins
Buffers
Gel Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Human platelet myosin. I. Purification by a rapid method applicable to other nonmuscle cells. / Pollard, Thomas D.; Thomas, Susan M.; Niederman, Richard.

In: Analytical Biochemistry, Vol. 60, No. 1, 1974, p. 258-266.

Research output: Contribution to journalArticle

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