Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones

D. T. Umetsu, J. S. Pober, H. H. Jabara, W. Fiers, E. J. Yunis, S. J. Burakoff, C. S. Reiss, R. S. Geha

Research output: Contribution to journalArticle

Abstract

Cultured human dermal fibroblasts treated with immune interferon express HLA-DR antigens. We report here that DR-positive fibroblasts present tetanus toxoid (TT) to autologous TT-specific monoclonal helper T cells vigorously depleted of monocytes by passage over Sephadex G10 columns followed by treatment with the monoclonal antibodies (mAb) OKM1 and Leu M1 plus complement. The extent of T cell proliferation in response to TT presented by DR-positive fibroblasts was similar to that elicited using monocytes as antigen-presenting cells. The proliferative response was TT dependent, antigen specific, depended upon DR expression by fibroblasts, appeared MHC restricted, and was completely blocked by mouse mAb to HLA-DR but not by mAb to HLA-A,B, or DQ. DR-positive fibroblasts pulsed with TT were similarly effective in antigen presentation. In summary, immune interferon-stimulated human dermal fibroblasts can substitute for classical antigen-presenting cells in antigen-specific proliferative responses. Since fibroblasts are a ubiquitous cell type in the body, they may play a significant role in the immunobiology of the host.

Original languageEnglish (US)
Pages (from-to)254-260
Number of pages7
JournalJournal of Clinical Investigation
Volume76
Issue number1
StatePublished - 1985

Fingerprint

Tetanus Toxoid
Clone Cells
Fibroblasts
T-Lymphocytes
Antigens
Skin
Monoclonal Antibodies
HLA-DR Antigens
Antigen-Presenting Cells
Interferon-gamma
Monocytes
HLA-DQ Antigens
HLA-A Antigens
HLA-B Antigens
Antigen Presentation
Helper-Inducer T-Lymphocytes
Cell Proliferation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Umetsu, D. T., Pober, J. S., Jabara, H. H., Fiers, W., Yunis, E. J., Burakoff, S. J., ... Geha, R. S. (1985). Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones. Journal of Clinical Investigation, 76(1), 254-260.

Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones. / Umetsu, D. T.; Pober, J. S.; Jabara, H. H.; Fiers, W.; Yunis, E. J.; Burakoff, S. J.; Reiss, C. S.; Geha, R. S.

In: Journal of Clinical Investigation, Vol. 76, No. 1, 1985, p. 254-260.

Research output: Contribution to journalArticle

Umetsu, DT, Pober, JS, Jabara, HH, Fiers, W, Yunis, EJ, Burakoff, SJ, Reiss, CS & Geha, RS 1985, 'Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones', Journal of Clinical Investigation, vol. 76, no. 1, pp. 254-260.
Umetsu DT, Pober JS, Jabara HH, Fiers W, Yunis EJ, Burakoff SJ et al. Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones. Journal of Clinical Investigation. 1985;76(1):254-260.
Umetsu, D. T. ; Pober, J. S. ; Jabara, H. H. ; Fiers, W. ; Yunis, E. J. ; Burakoff, S. J. ; Reiss, C. S. ; Geha, R. S. / Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones. In: Journal of Clinical Investigation. 1985 ; Vol. 76, No. 1. pp. 254-260.
@article{00cadc64bc9648a8bcab6726dbbde867,
title = "Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones",
abstract = "Cultured human dermal fibroblasts treated with immune interferon express HLA-DR antigens. We report here that DR-positive fibroblasts present tetanus toxoid (TT) to autologous TT-specific monoclonal helper T cells vigorously depleted of monocytes by passage over Sephadex G10 columns followed by treatment with the monoclonal antibodies (mAb) OKM1 and Leu M1 plus complement. The extent of T cell proliferation in response to TT presented by DR-positive fibroblasts was similar to that elicited using monocytes as antigen-presenting cells. The proliferative response was TT dependent, antigen specific, depended upon DR expression by fibroblasts, appeared MHC restricted, and was completely blocked by mouse mAb to HLA-DR but not by mAb to HLA-A,B, or DQ. DR-positive fibroblasts pulsed with TT were similarly effective in antigen presentation. In summary, immune interferon-stimulated human dermal fibroblasts can substitute for classical antigen-presenting cells in antigen-specific proliferative responses. Since fibroblasts are a ubiquitous cell type in the body, they may play a significant role in the immunobiology of the host.",
author = "Umetsu, {D. T.} and Pober, {J. S.} and Jabara, {H. H.} and W. Fiers and Yunis, {E. J.} and Burakoff, {S. J.} and Reiss, {C. S.} and Geha, {R. S.}",
year = "1985",
language = "English (US)",
volume = "76",
pages = "254--260",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "1",

}

TY - JOUR

T1 - Human dermal fibroblasts present tetanus toxoid antigen to antigen-specific T cell clones

AU - Umetsu, D. T.

AU - Pober, J. S.

AU - Jabara, H. H.

AU - Fiers, W.

AU - Yunis, E. J.

AU - Burakoff, S. J.

AU - Reiss, C. S.

AU - Geha, R. S.

PY - 1985

Y1 - 1985

N2 - Cultured human dermal fibroblasts treated with immune interferon express HLA-DR antigens. We report here that DR-positive fibroblasts present tetanus toxoid (TT) to autologous TT-specific monoclonal helper T cells vigorously depleted of monocytes by passage over Sephadex G10 columns followed by treatment with the monoclonal antibodies (mAb) OKM1 and Leu M1 plus complement. The extent of T cell proliferation in response to TT presented by DR-positive fibroblasts was similar to that elicited using monocytes as antigen-presenting cells. The proliferative response was TT dependent, antigen specific, depended upon DR expression by fibroblasts, appeared MHC restricted, and was completely blocked by mouse mAb to HLA-DR but not by mAb to HLA-A,B, or DQ. DR-positive fibroblasts pulsed with TT were similarly effective in antigen presentation. In summary, immune interferon-stimulated human dermal fibroblasts can substitute for classical antigen-presenting cells in antigen-specific proliferative responses. Since fibroblasts are a ubiquitous cell type in the body, they may play a significant role in the immunobiology of the host.

AB - Cultured human dermal fibroblasts treated with immune interferon express HLA-DR antigens. We report here that DR-positive fibroblasts present tetanus toxoid (TT) to autologous TT-specific monoclonal helper T cells vigorously depleted of monocytes by passage over Sephadex G10 columns followed by treatment with the monoclonal antibodies (mAb) OKM1 and Leu M1 plus complement. The extent of T cell proliferation in response to TT presented by DR-positive fibroblasts was similar to that elicited using monocytes as antigen-presenting cells. The proliferative response was TT dependent, antigen specific, depended upon DR expression by fibroblasts, appeared MHC restricted, and was completely blocked by mouse mAb to HLA-DR but not by mAb to HLA-A,B, or DQ. DR-positive fibroblasts pulsed with TT were similarly effective in antigen presentation. In summary, immune interferon-stimulated human dermal fibroblasts can substitute for classical antigen-presenting cells in antigen-specific proliferative responses. Since fibroblasts are a ubiquitous cell type in the body, they may play a significant role in the immunobiology of the host.

UR - http://www.scopus.com/inward/record.url?scp=0021967968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021967968&partnerID=8YFLogxK

M3 - Article

VL - 76

SP - 254

EP - 260

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 1

ER -