How does activation loop phosphorylation modulate catalytic activity in the cAMP-dependent protein kinase: A theoretical study

Yuhui Cheng, Yingkai Zhang, J. Andrew McCammon

Research output: Contribution to journalArticle

Abstract

Phosphorylation mediates the function of many proteins and enzymes. In the catalytic subunit of cAMP-dependent protein kinase, phosphorylation of Thr 197 in the activation loop strongly influences its catalytic activity. In order to provide theoretical understanding about this important regulatory process, classical molecular dynamics simulations and ab initio QM/MM calculations have been carried out on the wild-type PKA-Mg2 ATP-substrate complex and its dephosphorylated mutant, T197A. It was found that pThr 197 not only facilitates the phosphoryl transfer reaction by stabilizing the transition state through electrostatic interactions but also strongly affects its essential protein dynamics as well as the active site conformation.

Original languageEnglish (US)
Pages (from-to)672-683
Number of pages12
JournalProtein Science
Volume15
Issue number4
DOIs
StatePublished - Apr 2006

Fingerprint

Phosphorylation
Cyclic AMP-Dependent Protein Kinases
Catalyst activity
Catalytic Domain
Theoretical Models
Chemical activation
Molecular Dynamics Simulation
Coulomb interactions
Static Electricity
Conformations
Molecular dynamics
Proteins
Adenosine Triphosphate
Computer simulation
Substrates
Enzymes

Keywords

  • Collective motion
  • Molecular dynamics
  • Phosphorylation of Thr 197
  • Protein kinase A (PKA)
  • QM/MM calculations

ASJC Scopus subject areas

  • Biochemistry

Cite this

How does activation loop phosphorylation modulate catalytic activity in the cAMP-dependent protein kinase : A theoretical study. / Cheng, Yuhui; Zhang, Yingkai; McCammon, J. Andrew.

In: Protein Science, Vol. 15, No. 4, 04.2006, p. 672-683.

Research output: Contribution to journalArticle

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