Homogeneous cell printing on porous PCL/F127 tissue engineering scaffolds

Bin Wu, Shihao Li, Jia Shi, Sanjairaj Vijayavenkataraman, Wen Feng Lu, Dieter Trau, Jerry Ying Hsi Fuh

Research output: Contribution to journalArticle

Abstract

In tissue engineering, cell-laden scaffold has gradually replaced cell-less scaffold due to better biological performance. However, manual pipetting, the traditional cell seeding for cell-laden scaffold, leads to an imprecise and inhomogeneous cell distribution. As an alternative, micro-extrusion of cell-laden hydrogel achieves homogenous cell distribution, but causes high shear stress which is harmful to cells. To address this challenge, the objective of this study is to print cells on porous scaffold precisely without causing high shear stress to produce homogeneous cell-laden hybrid scaffold. Porous polycaprolactone scaffold fabricated through electro-hydrodynamic jetting was used as a representation. To improve scaffold hydrophilicity for better cell adhesion, 6% (w/w) Pluronic F127 was blended with polycaprolactone. HeLa cells, as a demonstration, were ejected on the scaffold fibers through piezoelectric inkjet printing. As a result, inkjet printing showed a more precise and homogeneous cell distribution and enhanced cell proliferation compared to manual pipetting (1.34- fold increase after 7 days). Furthermore, due to the low viscosity of cell solution, the average shear stress caused during inkjet printing was 1.79 kPa as opposed to 18 kPa of micro-extrusion, which is friendly to cells. In summary, through inkjet printing, homogeneous cell-laden hybrid scaffold could be fabricated with lower shear stress.

Original languageEnglish (US)
Article numbere00030
JournalBioprinting
Volume12
DOIs
StatePublished - Dec 1 2018

Fingerprint

UCON 50-HB-5100
Tissue Scaffolds
Printing
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
Shear stress
Polycaprolactone
Extrusion
Hybrid Cells
Cell adhesion
Hydrophilicity
Cell proliferation
Hydrogels

Keywords

  • Cell printing
  • E-Jetting
  • Piezoelectric inkjet printing
  • Porous tissue engineering scaffold

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Computer Science Applications

Cite this

Wu, B., Li, S., Shi, J., Vijayavenkataraman, S., Lu, W. F., Trau, D., & Fuh, J. Y. H. (2018). Homogeneous cell printing on porous PCL/F127 tissue engineering scaffolds. Bioprinting, 12, [e00030]. https://doi.org/10.1016/j.bprint.2018.e00030

Homogeneous cell printing on porous PCL/F127 tissue engineering scaffolds. / Wu, Bin; Li, Shihao; Shi, Jia; Vijayavenkataraman, Sanjairaj; Lu, Wen Feng; Trau, Dieter; Fuh, Jerry Ying Hsi.

In: Bioprinting, Vol. 12, e00030, 01.12.2018.

Research output: Contribution to journalArticle

Wu, B, Li, S, Shi, J, Vijayavenkataraman, S, Lu, WF, Trau, D & Fuh, JYH 2018, 'Homogeneous cell printing on porous PCL/F127 tissue engineering scaffolds', Bioprinting, vol. 12, e00030. https://doi.org/10.1016/j.bprint.2018.e00030
Wu B, Li S, Shi J, Vijayavenkataraman S, Lu WF, Trau D et al. Homogeneous cell printing on porous PCL/F127 tissue engineering scaffolds. Bioprinting. 2018 Dec 1;12. e00030. https://doi.org/10.1016/j.bprint.2018.e00030
Wu, Bin ; Li, Shihao ; Shi, Jia ; Vijayavenkataraman, Sanjairaj ; Lu, Wen Feng ; Trau, Dieter ; Fuh, Jerry Ying Hsi. / Homogeneous cell printing on porous PCL/F127 tissue engineering scaffolds. In: Bioprinting. 2018 ; Vol. 12.
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