Holographic microrefractometer

Hagay Shpaisman, Bhaskar Jyoti Krishnatreya, David G. Grier

    Research output: Contribution to journalArticle

    Abstract

    In-line holographic microscopy of micrometer-scale colloidal spheres yields heterodyne scattering patterns that may be interpreted with Lorenz-Mie theory to obtain precise time-resolved information on the refractive index of the suspending medium. We demonstrate this approach to spatially resolved refractometry with measurements on calibrated refractive index standards and use it to monitor chemical concentration in a microfluidic channel. Using commercially available colloidal spheres as probe particles and a standard video camera for detection yields values for the fluid's refractive index at the position of each probe particle in each holographic snapshot with a demonstrated resolution of 2 × 10 -3 refractive index units (RIU) and a potential resolution surpassing 10 -4 RIU. The combination of spatial resolution, temporal resolution, multi-point in situ access, and technical simplicity recommends this technique for cost-effective lab-on-a-chip applications.

    Original languageEnglish (US)
    Article number091102
    JournalApplied Physics Letters
    Volume101
    Issue number9
    DOIs
    StatePublished - Aug 27 2012

    Fingerprint

    refractivity
    probes
    Mie scattering
    temporal resolution
    micrometers
    spatial resolution
    cameras
    chips
    microscopy
    costs
    fluids
    scattering

    ASJC Scopus subject areas

    • Physics and Astronomy (miscellaneous)

    Cite this

    Shpaisman, H., Jyoti Krishnatreya, B., & Grier, D. G. (2012). Holographic microrefractometer. Applied Physics Letters, 101(9), [091102]. https://doi.org/10.1063/1.4747168

    Holographic microrefractometer. / Shpaisman, Hagay; Jyoti Krishnatreya, Bhaskar; Grier, David G.

    In: Applied Physics Letters, Vol. 101, No. 9, 091102, 27.08.2012.

    Research output: Contribution to journalArticle

    Shpaisman, H, Jyoti Krishnatreya, B & Grier, DG 2012, 'Holographic microrefractometer', Applied Physics Letters, vol. 101, no. 9, 091102. https://doi.org/10.1063/1.4747168
    Shpaisman H, Jyoti Krishnatreya B, Grier DG. Holographic microrefractometer. Applied Physics Letters. 2012 Aug 27;101(9). 091102. https://doi.org/10.1063/1.4747168
    Shpaisman, Hagay ; Jyoti Krishnatreya, Bhaskar ; Grier, David G. / Holographic microrefractometer. In: Applied Physics Letters. 2012 ; Vol. 101, No. 9.
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