HIV-1 and microvesicles from T cells share a common glycome, arguing for a common origin

Lakshmi Krishnamoorthy, Julian W. Bess, Alex B. Preston, Kunio Nagashima, Lara Mahal

Research output: Contribution to journalArticle

Abstract

HIV-1 is a master at deceiving the immune system and usurping host biosynthetic machinery. Although HIV-1 is coated with host-derived glycoproteins, only glycosylation of viral gp120 has been described. Here we use lectin microarray technology to analyze the glycome of intact HIV-1 virions. We show that the glycan coat of human T cell line-derived HIV-1 matches that of native immunomodulatory microvesicles. The carbohydrate composition of both virus and microvesicles is cell-line dependent, which suggests a mechanism to rapidly camouflage the virus within the host. In addition, binding of both virus and microvesicles to antiviral lectins is enriched over the host cell, raising concern about targeting these glycans for therapeutics. This work also sheds light on the binding of HIV-1 to galectin-1, an important human immune lectin. Overall, our work strongly supports the theory that HIV-1 co-opts the exocytic pathway of microvesicles, thus potentially explaining why eliciting a protective antiviral immune response is difficult.

Original languageEnglish (US)
Pages (from-to)244-250
Number of pages7
JournalNature Chemical Biology
Volume5
Issue number4
DOIs
StatePublished - Apr 2 2009

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HIV-1
T-Lymphocytes
Lectins
Antiviral Agents
Polysaccharides
Viruses
Virus Attachment
Cell Line
Glycosylation
Virion
Immune System
Glycoproteins
Carbohydrates
Technology
Therapeutics

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

HIV-1 and microvesicles from T cells share a common glycome, arguing for a common origin. / Krishnamoorthy, Lakshmi; Bess, Julian W.; Preston, Alex B.; Nagashima, Kunio; Mahal, Lara.

In: Nature Chemical Biology, Vol. 5, No. 4, 02.04.2009, p. 244-250.

Research output: Contribution to journalArticle

Krishnamoorthy, Lakshmi ; Bess, Julian W. ; Preston, Alex B. ; Nagashima, Kunio ; Mahal, Lara. / HIV-1 and microvesicles from T cells share a common glycome, arguing for a common origin. In: Nature Chemical Biology. 2009 ; Vol. 5, No. 4. pp. 244-250.
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