Histological and three-dimensional evaluation of osseointegration to nanostructured calcium phosphate-coated implants

Ryo Jimbo, Paulo Coelho, Stefan Vandeweghe, Humberto Osvaldo Schwartz-Filho, Mariko Hayashi, Daisuke Ono, Martin Andersson, Ann Wennerberg

Research output: Contribution to journalArticle

Abstract

Nanostructures on implant surfaces have been shown to enhance osseointegration; however, commonly used evaluation techniques are probably not sufficiently sensitive to fully determine the effects of this process. This study aimed to observe the osseointegration properties of nanostructured calcium phosphate (CaP)-coated implants, by using a combination of three-dimensional imaging and conventional histology. Titanium implants were coated with stable CaP nanoparticles using an immersion technique followed by heat treatment. Uncoated implants were used as the control. After topographical and chemical characterizations, implants were inserted into the rabbit femur. After 2 and 4 weeks, the samples were retrieved for micro-computed tomography and histomorphometric evaluation. Scanning electron microscopy evaluation indicated that the implant surface was modified at the nanoscale by CaP to obtain surface textured with rod-shaped structures. Relative to the control, the bone-to-implant contact for the CaP-coated implant was significantly higher at 4 weeks after the implant surgery. Further, corresponding 3-D images showed active bone formation surrounding the implant. 3-D quantification and 2-D histology demonstrated statistical correlation; moreover, 3-D quantification indicated a statistical decrease in bone density in the non-coated control implant group between 2 and 4 weeks after the surgery. The application of 3-D evaluation further clarified the temporal characteristics and biological reaction of implants in bone.

Original languageEnglish (US)
Pages (from-to)4229-4234
Number of pages6
JournalActa Biomaterialia
Volume7
Issue number12
DOIs
StatePublished - Dec 2011

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Osseointegration
Calcium phosphate
Bone
Histology
Three-Dimensional Imaging
Surgery
Bone and Bones
Nanostructures
Immersion
Titanium
Osteogenesis
Electron Scanning Microscopy
Bone Density
Femur
Nanoparticles
Tomography
Hot Temperature
Heat treatment
Rabbits
Imaging techniques

Keywords

  • Bone implant interactions
  • Micro-CT
  • Nano structures
  • Surface chemistry

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Histological and three-dimensional evaluation of osseointegration to nanostructured calcium phosphate-coated implants. / Jimbo, Ryo; Coelho, Paulo; Vandeweghe, Stefan; Schwartz-Filho, Humberto Osvaldo; Hayashi, Mariko; Ono, Daisuke; Andersson, Martin; Wennerberg, Ann.

In: Acta Biomaterialia, Vol. 7, No. 12, 12.2011, p. 4229-4234.

Research output: Contribution to journalArticle

Jimbo, R, Coelho, P, Vandeweghe, S, Schwartz-Filho, HO, Hayashi, M, Ono, D, Andersson, M & Wennerberg, A 2011, 'Histological and three-dimensional evaluation of osseointegration to nanostructured calcium phosphate-coated implants', Acta Biomaterialia, vol. 7, no. 12, pp. 4229-4234. https://doi.org/10.1016/j.actbio.2011.07.017
Jimbo, Ryo ; Coelho, Paulo ; Vandeweghe, Stefan ; Schwartz-Filho, Humberto Osvaldo ; Hayashi, Mariko ; Ono, Daisuke ; Andersson, Martin ; Wennerberg, Ann. / Histological and three-dimensional evaluation of osseointegration to nanostructured calcium phosphate-coated implants. In: Acta Biomaterialia. 2011 ; Vol. 7, No. 12. pp. 4229-4234.
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