Hippo/YAP-mediated rigidity-dependent motor neuron differentiation of human pluripotent stem cells

Yubing Sun, Koh Meng Aw Yong, Luis G. Villa-Diaz, Xiaoli Zhang, Weiqiang Chen, Renee Philson, Shinuo Weng, Haoxing Xu, Paul H. Krebsbach, Jianping Fu

Research output: Contribution to journalArticle

Abstract

Our understanding of the intrinsic mechanosensitive properties of human pluripotent stem cells (hPSCs), in particular the effects that the physical microenvironment has on their differentiation, remains elusive. Here, we show that neural induction and caudalization of hPSCs can be accelerated by using a synthetic microengineered substrate system consisting of poly(dimethylsiloxane) micropost arrays (PMAs) with tunable mechanical rigidities. The purity and yield of functional motor neurons derived from hPSCs within 23 days of culture using soft PMAs were improved more than fourfold and tenfold, respectively, compared with coverslips or rigid PMAs. Mechanistic studies revealed a multi-targeted mechanotransductive process involving Smad phosphorylation and nucleocytoplasmic shuttling, regulated by rigidity-dependent Hippo/YAP activities and actomyosin cytoskeleton integrity and contractility. Our findings suggest that substrate rigidity is an important biophysical cue influencing neural induction and subtype specification, and that microengineered substrates can thus serve as a promising platform for large-scale culture of hPSCs.

Original languageEnglish (US)
Pages (from-to)599-604
Number of pages6
JournalNature Materials
Volume13
Issue number6
DOIs
StatePublished - 2014

Fingerprint

stem cells
Stem cells
neurons
rigidity
Rigidity
Neurons
Polydimethylsiloxane
Cell culture
induction
Substrates
Actomyosin
phosphorylation
Phosphorylation
cues
integrity
specifications
purity
platforms
Specifications
baysilon

ASJC Scopus subject areas

  • Mechanical Engineering
  • Mechanics of Materials
  • Condensed Matter Physics
  • Materials Science(all)
  • Chemistry(all)

Cite this

Sun, Y., Yong, K. M. A., Villa-Diaz, L. G., Zhang, X., Chen, W., Philson, R., ... Fu, J. (2014). Hippo/YAP-mediated rigidity-dependent motor neuron differentiation of human pluripotent stem cells. Nature Materials, 13(6), 599-604. https://doi.org/10.1038/nmat3945

Hippo/YAP-mediated rigidity-dependent motor neuron differentiation of human pluripotent stem cells. / Sun, Yubing; Yong, Koh Meng Aw; Villa-Diaz, Luis G.; Zhang, Xiaoli; Chen, Weiqiang; Philson, Renee; Weng, Shinuo; Xu, Haoxing; Krebsbach, Paul H.; Fu, Jianping.

In: Nature Materials, Vol. 13, No. 6, 2014, p. 599-604.

Research output: Contribution to journalArticle

Sun, Y, Yong, KMA, Villa-Diaz, LG, Zhang, X, Chen, W, Philson, R, Weng, S, Xu, H, Krebsbach, PH & Fu, J 2014, 'Hippo/YAP-mediated rigidity-dependent motor neuron differentiation of human pluripotent stem cells', Nature Materials, vol. 13, no. 6, pp. 599-604. https://doi.org/10.1038/nmat3945
Sun, Yubing ; Yong, Koh Meng Aw ; Villa-Diaz, Luis G. ; Zhang, Xiaoli ; Chen, Weiqiang ; Philson, Renee ; Weng, Shinuo ; Xu, Haoxing ; Krebsbach, Paul H. ; Fu, Jianping. / Hippo/YAP-mediated rigidity-dependent motor neuron differentiation of human pluripotent stem cells. In: Nature Materials. 2014 ; Vol. 13, No. 6. pp. 599-604.
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