HIM-10 is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes

Mary Howe, Kent L. McDonald, Donna Albertson, Barbara J. Meyer

Research output: Contribution to journalArticle

Abstract

Macromolecular structures called kinetochores attach and move chromosomes within the spindle during chromosome segregation. Using electron microscopy, we identified a structure on the holocentric mitotic and meiotic chromosomes of Caenorhabditis elegans that resembles the mammalian kinetochore. This structure faces the poles on mitotic chromosomes but encircles meiotic chromosomes. Worm kinetochores require the evolutionarily conserved HIM-10 protein for their structure and function. HIM-10 localizes to the kinetochores and mediates attachment of chromosomes to the spindle. Depletion of HIM-10 disrupts kinetochore structure, causes a failure of bipolar spindle attachment, and results in chromosome nondisjunction. HIM-10 is related to the Nuf2 kinetochore proteins conserved from yeast to humans. Thus, the extended kinetochores characteristic of C. elegans holocentric chromosomes provide a guide to the structure, molecular architecture, and function of conventional kinetochores.

Original languageEnglish (US)
Pages (from-to)1227-1238
Number of pages12
JournalJournal of Cell Biology
Volume153
Issue number6
DOIs
StatePublished - Jun 11 2001

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Kinetochores
Caenorhabditis elegans
Chromosomes
Chromosome Segregation
Fungal Proteins
Molecular Structure
Electron Microscopy

Keywords

  • Caenorhabditis elegans
  • Holocentric chromosomes
  • Kinetochore
  • Meiosis
  • Mitosis

ASJC Scopus subject areas

  • Cell Biology

Cite this

HIM-10 is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes. / Howe, Mary; McDonald, Kent L.; Albertson, Donna; Meyer, Barbara J.

In: Journal of Cell Biology, Vol. 153, No. 6, 11.06.2001, p. 1227-1238.

Research output: Contribution to journalArticle

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