High-throughput sorting of eggs for synchronization of: C. elegans in a microfluidic spiral chip

Samuel Sofela, Sarah Sahloul, Mehdi Rafeie, Taehong Kwon, Jongyoon Han, Majid Ebrahimi Warkiani, Rafael Song

Research output: Contribution to journalArticle

Abstract

In this study, we report the use of a high-throughput microfluidic spiral chip to screen out eggs from a mixed age nematode population, which can subsequently be cultured to a desired developmental stage. For the sorting of a mixture containing three different developmental stages, eggs, L1 and L4, we utilized a microfluidic spiral chip with a trapezoidal channel to obtain a sorting efficiency of above 97% and a sample purity (SP) of above 80% for eggs at different flow rates up to 10 mL min-1. The result demonstrated a cost-effective, simple, and highly efficient method for synchronizing C. elegans at a high throughput (∼4200 organisms per min at 6 mL min-1), while eliminating challenges such as clogging and non-reusability of membrane-based filtration. Due to its simplicity, our method can be easily adopted in the C. elegans research community.

Original languageEnglish (US)
Pages (from-to)679-687
Number of pages9
JournalLab on a Chip
Volume18
Issue number4
DOIs
StatePublished - Jan 1 2018

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Microfluidics
Sorting
Eggs
Synchronization
Throughput
Flow rate
Membranes
Costs
Costs and Cost Analysis
Research
Population

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

High-throughput sorting of eggs for synchronization of : C. elegans in a microfluidic spiral chip. / Sofela, Samuel; Sahloul, Sarah; Rafeie, Mehdi; Kwon, Taehong; Han, Jongyoon; Warkiani, Majid Ebrahimi; Song, Rafael.

In: Lab on a Chip, Vol. 18, No. 4, 01.01.2018, p. 679-687.

Research output: Contribution to journalArticle

Sofela, S, Sahloul, S, Rafeie, M, Kwon, T, Han, J, Warkiani, ME & Song, R 2018, 'High-throughput sorting of eggs for synchronization of: C. elegans in a microfluidic spiral chip', Lab on a Chip, vol. 18, no. 4, pp. 679-687. https://doi.org/10.1039/c7lc00998d
Sofela, Samuel ; Sahloul, Sarah ; Rafeie, Mehdi ; Kwon, Taehong ; Han, Jongyoon ; Warkiani, Majid Ebrahimi ; Song, Rafael. / High-throughput sorting of eggs for synchronization of : C. elegans in a microfluidic spiral chip. In: Lab on a Chip. 2018 ; Vol. 18, No. 4. pp. 679-687.
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