High-Resolution 1H NMR Study of the Solution Structure of Alamethicin

Gennaro Esposito, John A. Carver, Jonathan Boyd, Iain D. Campbell

    Research output: Contribution to journalArticle

    Abstract

    A 1H NMR study of the peptide alamethicin, which forms voltage-gated ion channels in membranes, is described. The molecule was studied in methanol as a function of temperature and pH. A complete assignment of the spectra is given, including several stereospecific assignments. Alamethicin was found to have a structure substantially similar to the crystal although, in solution, the C-terminal dipeptide adopts a somewhat extended conformation. The overall conformation was insensitive to the ionization of the side chain of the only ionizable group, Glu-18.

    Original languageEnglish (US)
    Pages (from-to)1043-1050
    Number of pages8
    JournalBiochemistry
    Volume26
    Issue number4
    DOIs
    StatePublished - Jan 1 1987

    Fingerprint

    Alamethicin
    Conformations
    Nuclear magnetic resonance
    Dipeptides
    Ion Channels
    Ionization
    Methanol
    Membranes
    Peptides
    Crystals
    Molecules
    Temperature
    Electric potential
    Proton Magnetic Resonance Spectroscopy

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Esposito, G., Carver, J. A., Boyd, J., & Campbell, I. D. (1987). High-Resolution 1H NMR Study of the Solution Structure of Alamethicin. Biochemistry, 26(4), 1043-1050. https://doi.org/10.1021/bi00378a010

    High-Resolution 1H NMR Study of the Solution Structure of Alamethicin. / Esposito, Gennaro; Carver, John A.; Boyd, Jonathan; Campbell, Iain D.

    In: Biochemistry, Vol. 26, No. 4, 01.01.1987, p. 1043-1050.

    Research output: Contribution to journalArticle

    Esposito, G, Carver, JA, Boyd, J & Campbell, ID 1987, 'High-Resolution 1H NMR Study of the Solution Structure of Alamethicin', Biochemistry, vol. 26, no. 4, pp. 1043-1050. https://doi.org/10.1021/bi00378a010
    Esposito, Gennaro ; Carver, John A. ; Boyd, Jonathan ; Campbell, Iain D. / High-Resolution 1H NMR Study of the Solution Structure of Alamethicin. In: Biochemistry. 1987 ; Vol. 26, No. 4. pp. 1043-1050.
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