High-performance gel permeation chromatography of glycosaminoglycans column calibration by gel electrophoresis

Daniel M. Hittner, Mary Cowman

Research output: Contribution to journalArticle

Abstract

High-performance gel permeation chromatography and sensitivity-enhanced polyacrylamide gel electrophoresis (SE-PAGE) have been combined to investigate the chromatographic properties of structurally related glycosaminoglycans. Enzymatically digested samples of hyaluronate, chondroitin 4-sulfate and dermatan sulfate were fractionated on a column series of TSK G4000SW and TSK G2000SW, eluted with 0.15 M sodium chloride. Isolated fractions were subjected to sensitivity-enhanced polyacrylamide gel eletrophoresis for determination of molecular weight. These procedures allowed the rapid development of column calibration profiles for each glycosaminoglycan, under a given set of chromatographic conditions. The profiles obtained in 0.15 M sodium chloride yielded the following data: (1) at equal degrees of polymerization, hyaluronic acid chains have an apparently smaller hydrodynamic radius than the sulfated polymers, and (2) at equal molecular weights, all three glycosaminoglycans have approximately equal hydrodynamic radii.

Original languageEnglish (US)
Pages (from-to)149-158
Number of pages10
JournalJournal of Chromatography A
Volume402
Issue numberC
DOIs
StatePublished - 1987

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Gel permeation chromatography
Glycosaminoglycans
Electrophoresis
Calibration
Gel Chromatography
Gels
Hydrodynamics
Sodium Chloride
Molecular Weight
Molecular weight
Dermatan Sulfate
Chondroitin Sulfates
Hyaluronic Acid
Polymerization
Polyacrylamide Gel Electrophoresis
Polymers
polyacrylamide gels

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

High-performance gel permeation chromatography of glycosaminoglycans column calibration by gel electrophoresis. / Hittner, Daniel M.; Cowman, Mary.

In: Journal of Chromatography A, Vol. 402, No. C, 1987, p. 149-158.

Research output: Contribution to journalArticle

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