Heterotopic Transplantations Reveal Environmental Influences on Interneuron Diversity and Maturation

Giulia Quattrocolo, Gordon Fishell, Timothy J. Petros

    Research output: Contribution to journalArticle

    Abstract

    During embryogenesis, neural progenitors in the ganglionic eminences give rise to diverse GABAergic interneuron subtypes that populate all forebrain regions. The extent to which these cells are genetically predefined or determined by postmigratory environmental cues remains unknown. To address this question, we performed homo- and heterotopic transplantation of early postnatal MGE-derived cortical and hippocampal interneurons. Grafted cells migrated, and displayed neurochemical, electrophysiological, morphological, and neurochemical profiles similar to endogenous interneurons. Our results indicate that the host environment regulates the proportion of interneuron classes in the brain region. However, some specific interneuron subtypes retain characteristics representative of their donor brain regions. GABAergic interneurons are an extremely heterogeneous cell population, but the mechanisms directing this diversity remain unclear. Quattrocolo et al. challenged interneuron precursors in new environments via heterotopic transplantations in order to investigate how the environment influences interneuron diversity.

    Original languageEnglish (US)
    Pages (from-to)721-731
    Number of pages11
    JournalCell Reports
    Volume21
    Issue number3
    DOIs
    StatePublished - Oct 17 2017

    Fingerprint

    Heterotopic Transplantation
    Interneurons
    Brain
    Cells
    Prosencephalon
    Embryonic Development
    Cues

    Keywords

    • interneurons
    • neurodevelopment
    • nNos
    • parvalbumin
    • somatostatin
    • transplantation

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Heterotopic Transplantations Reveal Environmental Influences on Interneuron Diversity and Maturation. / Quattrocolo, Giulia; Fishell, Gordon; Petros, Timothy J.

    In: Cell Reports, Vol. 21, No. 3, 17.10.2017, p. 721-731.

    Research output: Contribution to journalArticle

    Quattrocolo, Giulia ; Fishell, Gordon ; Petros, Timothy J. / Heterotopic Transplantations Reveal Environmental Influences on Interneuron Diversity and Maturation. In: Cell Reports. 2017 ; Vol. 21, No. 3. pp. 721-731.
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