Heterochromatic position effect at the rosy locus of Drosophila melanogaster: cytological, genetic and biochemical characterization.

Christine Rushlow, A. Chovnick

Research output: Contribution to journalArticle

Abstract

This report describes cytological, genetic and biochemical studies designed to characterize two gamma-radiation induced, apparent "underproducer" variants of the rosy locus (ry:3-52.0), ryps1149 and ryps11136. The following observations provide a compelling basis for their diagnosis as heterochromatic position effect variants. They are associated with rearrangements that place heterochromatin adjacent to the rosy region of chromosome 3 (87D). The effect of these mutations on rosy locus expression is subject to modification by abnormal Y chromosome content. The rearrangement alters only the expression of the rosy allele on the same chromosome (cis-acting). The Y chromosome modification is only on the position-affected allele's expression. The recessive lethality associated with the rearrangements relate to specific rosy region vital loci, and for ryps11136, the lethality is not Y chromosome modified. The peptide product of the position-affected allele is qualitatively normal by several criteria. Heterozygous deletion of 87E2-F2 is a suppressor of the rosy position effect. The rosy position effect on XDH production may be assayed in whole larvae and larval fat body tissue as well as in adults.

Original languageEnglish (US)
Pages (from-to)589-602
Number of pages14
JournalGenetics
Volume108
Issue number3
StatePublished - Nov 1984

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Y Chromosome
Drosophila melanogaster
Molecular Biology
Alleles
Chromosomes, Human, Pair 3
Fat Body
Heterochromatin
Gamma Rays
Larva
Chromosomes
Peptides
Mutation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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Heterochromatic position effect at the rosy locus of Drosophila melanogaster : cytological, genetic and biochemical characterization. / Rushlow, Christine; Chovnick, A.

In: Genetics, Vol. 108, No. 3, 11.1984, p. 589-602.

Research output: Contribution to journalArticle

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