Hepatitis B virus genotypes and HBsAg subtypes in refugees and injection drug users in the United States determined by LiPA and monoclonal EIA

P. D. Swenson, C. Van Geyt, E. Russell Alexander, Holly Hagan, J. M. Freitag-Koontz, S. Wilson, H. Norder, L. O. Magnius, L. Stuyver

Research output: Contribution to journalArticle

Abstract

Hepatitis B virus (HBV) genotyping and hepatitis B surface antigen (HBsAg) subtyping were carried out on sera from 196 HBsAg-positive patients, including 151 refugees entering the United States and 45 injection drug users in Seattle. HBsAg subtyping was performed by enzyme immunoassay (EIA) using a panel of monoclonal antibodies and the HBV genotype was determined by polymerase chain reaction (PCR) followed by detection of amplified HBV DNA by a reverse-phase hybridization line probe assay (LiPA) using genotype-specific probes. HBV DNA was detected by PCR in 155 (79%) of the 196 sera and all 155 were genotyped by LiPA. Samples from Southeast Asia were predominantly genotype B/subtype ayw1 and genotype C/adr; samples from the former Soviet Union and eastern Europe were mostly genotype D/ayw2 and genotype D/ayw3; samples from east Africa were mainly genotype A/adw2 and genotype D/ayw2; and samples from injection drug users were mostly genotype D/ayw3 and genotype A/adw2. Some strains of ayw3 gave atypical monoclonal antibody reactivity patterns in the subtyping assay due to a Val/Ala instead of a Thr at amino acid residue 118 and a Thr instead of a Met at residue 125. A strain of ayw2 also gave an atypical monoclonal antibody reactivity pattern due to an Ala instead of a Thr at amino acid residue 123. LiPA genotyping and monoclonal EIA subtyping can provide useful information for epidemiological studies.

Original languageEnglish (US)
Pages (from-to)305-311
Number of pages7
JournalJournal of Medical Virology
Volume64
Issue number3
DOIs
StatePublished - 2001

Fingerprint

Refugees
Hepatitis B Surface Antigens
Drug Users
Immunoenzyme Techniques
Hepatitis B virus
Genotype
Injections
Monoclonal Antibodies
Amino Acids
Eastern Europe
Eastern Africa
Polymerase Chain Reaction
Southeastern Asia
USSR
DNA
Serum
Epidemiologic Studies

Keywords

  • Genotypes
  • Hepatitis B surface antigen
  • Hepatitis B virus
  • Line probe assay
  • Monoclonal antibody
  • Subtypes

ASJC Scopus subject areas

  • Virology

Cite this

Hepatitis B virus genotypes and HBsAg subtypes in refugees and injection drug users in the United States determined by LiPA and monoclonal EIA. / Swenson, P. D.; Van Geyt, C.; Russell Alexander, E.; Hagan, Holly; Freitag-Koontz, J. M.; Wilson, S.; Norder, H.; Magnius, L. O.; Stuyver, L.

In: Journal of Medical Virology, Vol. 64, No. 3, 2001, p. 305-311.

Research output: Contribution to journalArticle

Swenson, PD, Van Geyt, C, Russell Alexander, E, Hagan, H, Freitag-Koontz, JM, Wilson, S, Norder, H, Magnius, LO & Stuyver, L 2001, 'Hepatitis B virus genotypes and HBsAg subtypes in refugees and injection drug users in the United States determined by LiPA and monoclonal EIA', Journal of Medical Virology, vol. 64, no. 3, pp. 305-311. https://doi.org/10.1002/jmv.1051
Swenson, P. D. ; Van Geyt, C. ; Russell Alexander, E. ; Hagan, Holly ; Freitag-Koontz, J. M. ; Wilson, S. ; Norder, H. ; Magnius, L. O. ; Stuyver, L. / Hepatitis B virus genotypes and HBsAg subtypes in refugees and injection drug users in the United States determined by LiPA and monoclonal EIA. In: Journal of Medical Virology. 2001 ; Vol. 64, No. 3. pp. 305-311.
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