Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota

Soo Ching Lee, Mei San Tang, Yvonne A L Lim, Seow Huey Choy, Zachary D. Kurtz, Laura M. Cox, Uma Mahesh Gundra, Ilseung Cho, Richard Bonneau, Martin J. Blaser, Kek Heng Chua, P'ng Loke

Research output: Contribution to journalArticle

Abstract

Soil-transmitted helminths colonize more than 1.5 billion people worldwide, yet little is known about how they interact with bacterial communities in the gut microbiota. Differences in the gut microbiota between individuals living in developed and developing countries may be partly due to the presence of helminths, since they predominantly infect individuals from developing countries, such as the indigenous communities in Malaysia we examine in this work. We compared the composition and diversity of bacterial communities from the fecal microbiota of 51 people from two villages in Malaysia, of which 36 (70.6%) were infected by helminths. The 16S rRNA V4 region was sequenced at an average of nineteen thousand sequences per samples. Helminth-colonized individuals had greater species richness and number of observed OTUs with enrichment of Paraprevotellaceae, especially with Trichuris infection. We developed a new approach of combining centered log-ratio (clr) transformation for OTU relative abundances with sparse Partial Least Squares Discriminant Analysis (sPLS-DA) to enable more robust predictions of OTU interrelationships. These results suggest that helminths may have an impact on the diversity, bacterial community structure and function of the gut microbiota.

Original languageEnglish (US)
Article numbere2880
JournalPLoS Neglected Tropical Diseases
Volume8
Issue number5
DOIs
StatePublished - 2014

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Helminths
Malaysia
Developing Countries
Trichuris
Bacterial Structures
Microbiota
Discriminant Analysis
Least-Squares Analysis
Developed Countries
Soil
Gastrointestinal Microbiome
Infection

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Lee, S. C., Tang, M. S., Lim, Y. A. L., Choy, S. H., Kurtz, Z. D., Cox, L. M., ... Loke, P. (2014). Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota. PLoS Neglected Tropical Diseases, 8(5), [e2880]. https://doi.org/10.1371/journal.pntd.0002880

Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota. / Lee, Soo Ching; Tang, Mei San; Lim, Yvonne A L; Choy, Seow Huey; Kurtz, Zachary D.; Cox, Laura M.; Gundra, Uma Mahesh; Cho, Ilseung; Bonneau, Richard; Blaser, Martin J.; Chua, Kek Heng; Loke, P'ng.

In: PLoS Neglected Tropical Diseases, Vol. 8, No. 5, e2880, 2014.

Research output: Contribution to journalArticle

Lee, SC, Tang, MS, Lim, YAL, Choy, SH, Kurtz, ZD, Cox, LM, Gundra, UM, Cho, I, Bonneau, R, Blaser, MJ, Chua, KH & Loke, P 2014, 'Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota', PLoS Neglected Tropical Diseases, vol. 8, no. 5, e2880. https://doi.org/10.1371/journal.pntd.0002880
Lee, Soo Ching ; Tang, Mei San ; Lim, Yvonne A L ; Choy, Seow Huey ; Kurtz, Zachary D. ; Cox, Laura M. ; Gundra, Uma Mahesh ; Cho, Ilseung ; Bonneau, Richard ; Blaser, Martin J. ; Chua, Kek Heng ; Loke, P'ng. / Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota. In: PLoS Neglected Tropical Diseases. 2014 ; Vol. 8, No. 5.
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