Helical complexes of polyriboinosinic acid with copolymers of polyribocytidylic acid containing inosine, adenosine and uridine residues

Amy C. Wang, Neville R. Kallenbach

Research output: Contribution to journalArticle

Abstract

The consequences of incorporating non-complementary residues into the poly (I) · poly (C) helix have been investigated. Complexes of poly (I) and copolymers of C with different mole-ratios of I, A and U residues have been prepared and denatured in a variety of solvents. The results of both denaturation and analysis of the stoichiometry of the reactions suggest that in poly (I)· poly (C, Ix) complexes, the I residues are excluded from the helix matrix, whereas in the poly (I) · poly (C, Ux) and poly (I) · poly (C, Ax) systems the minor component bases are retained. Preliminaries to a quantitative analysis of the transition data are presented, permitting rough estimates of the difference in stability between poly (I) · poly (C) and poly (I) · poly (U) or poly (I) · poly (A) pairs in these complexes-the results being 1.7 kcal./mole and 1.3 kcal./mole, respectively. The differences in behavior of poly (I) · poly (C, I) complexes are found to be most evident in the presence of 8 m-urea.

Original languageEnglish (US)
Pages (from-to)591-607
Number of pages17
JournalJournal of Molecular Biology
Volume62
Issue number3
DOIs
StatePublished - Dec 28 1971

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Poly C
Poly I-C
Inosine
Uridine
Adenosine
Poly I
Acids
Poly U
Poly A
Urea

ASJC Scopus subject areas

  • Virology

Cite this

Helical complexes of polyriboinosinic acid with copolymers of polyribocytidylic acid containing inosine, adenosine and uridine residues. / Wang, Amy C.; Kallenbach, Neville R.

In: Journal of Molecular Biology, Vol. 62, No. 3, 28.12.1971, p. 591-607.

Research output: Contribution to journalArticle

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