H4K20me1 Contributes to Downregulation of X-Linked Genes for C. elegans Dosage Compensation

Anne Vielle, Jackie Lang, Yan Dong, Sevinc Ercan, Chitra Kotwaliwale, Andreas Rechtsteiner, Alex Appert, Q. Brent Chen, Andrea Dose, Thea Egelhofer, Hiroshi Kimura, Przemyslaw Stempor, Abby Dernburg, Jason D. Lieb, Susan Strome, Julie Ahringer

Research output: Contribution to journalArticle

Abstract

The Caenorhabditis elegans dosage compensation complex (DCC) equalizes X-chromosome gene dosage between XO males and XX hermaphrodites by two-fold repression of X-linked gene expression in hermaphrodites. The DCC localizes to the X chromosomes in hermaphrodites but not in males, and some subunits form a complex homologous to condensin. The mechanism by which the DCC downregulates gene expression remains unclear. Here we show that the DCC controls the methylation state of lysine 20 of histone H4, leading to higher H4K20me1 and lower H4K20me3 levels on the X chromosomes of XX hermaphrodites relative to autosomes. We identify the PR-SET7 ortholog SET-1 and the Suv4-20 ortholog SET-4 as the major histone methyltransferases for monomethylation and di/trimethylation of H4K20, respectively, and provide evidence that X-chromosome enrichment of H4K20me1 involves inhibition of SET-4 activity on the X. RNAi knockdown of set-1 results in synthetic lethality with dosage compensation mutants and upregulation of X-linked gene expression, supporting a model whereby H4K20me1 functions with the condensin-like C. elegans DCC to repress transcription of X-linked genes. H4K20me1 is important for mitotic chromosome condensation in mammals, suggesting that increased H4K20me1 on the X may restrict access of the transcription machinery to X-linked genes via chromatin compaction.

Original languageEnglish (US)
Article numbere1002933
JournalPLoS Genetics
Volume8
Issue number9
DOIs
StatePublished - Sep 2012

Fingerprint

X-Linked Genes
Down-Regulation
X chromosome
hermaphrodite
X Chromosome
chromosome
gene
dosage
Caenorhabditis elegans
Gene Expression
genes
gene expression
histones
transcription (genetics)
Gene Dosage
gene dosage
RNA Interference
autosomes
Histones
Methylation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Vielle, A., Lang, J., Dong, Y., Ercan, S., Kotwaliwale, C., Rechtsteiner, A., ... Ahringer, J. (2012). H4K20me1 Contributes to Downregulation of X-Linked Genes for C. elegans Dosage Compensation. PLoS Genetics, 8(9), [e1002933]. https://doi.org/10.1371/journal.pgen.1002933

H4K20me1 Contributes to Downregulation of X-Linked Genes for C. elegans Dosage Compensation. / Vielle, Anne; Lang, Jackie; Dong, Yan; Ercan, Sevinc; Kotwaliwale, Chitra; Rechtsteiner, Andreas; Appert, Alex; Chen, Q. Brent; Dose, Andrea; Egelhofer, Thea; Kimura, Hiroshi; Stempor, Przemyslaw; Dernburg, Abby; Lieb, Jason D.; Strome, Susan; Ahringer, Julie.

In: PLoS Genetics, Vol. 8, No. 9, e1002933, 09.2012.

Research output: Contribution to journalArticle

Vielle, A, Lang, J, Dong, Y, Ercan, S, Kotwaliwale, C, Rechtsteiner, A, Appert, A, Chen, QB, Dose, A, Egelhofer, T, Kimura, H, Stempor, P, Dernburg, A, Lieb, JD, Strome, S & Ahringer, J 2012, 'H4K20me1 Contributes to Downregulation of X-Linked Genes for C. elegans Dosage Compensation', PLoS Genetics, vol. 8, no. 9, e1002933. https://doi.org/10.1371/journal.pgen.1002933
Vielle, Anne ; Lang, Jackie ; Dong, Yan ; Ercan, Sevinc ; Kotwaliwale, Chitra ; Rechtsteiner, Andreas ; Appert, Alex ; Chen, Q. Brent ; Dose, Andrea ; Egelhofer, Thea ; Kimura, Hiroshi ; Stempor, Przemyslaw ; Dernburg, Abby ; Lieb, Jason D. ; Strome, Susan ; Ahringer, Julie. / H4K20me1 Contributes to Downregulation of X-Linked Genes for C. elegans Dosage Compensation. In: PLoS Genetics. 2012 ; Vol. 8, No. 9.
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