H-2L(d) antigen encoded by a recombinant retrovirus genome is expressed on the surface of infected cells

J. H. Weis, Carol Reiss, R. C. Mulligan

Research output: Contribution to journalArticle

Abstract

A recombinant murine retrovirus was constructed which contains, within its genome, a truncated version of the gene encoding the murine H-2L(d) major histocompatibility antigen. The H-2L(d) gene, which was inserted 3' of the env splice acceptor site in the recombinant retrovirus MSV-neo, lacked the 5' promoter and TATA sequences and the 3' transcription termination and polyadenylate addition sites of the normal H-2L(d) gene. Transfection of the MSV-neo/H-2L(d) plasmid (pLTV-11) into Y-2 cells resulted in the production of the transmissible recombinant retrovirus LTV-11. Cells infected with LTV-11 virus were resistant to the eucaryotic antibiotic G418 and expressed H-2L(d) on the cell surface. These infected cells contained a viral RNA species which possessed both the H-2L(d) and the neomycin resistance gene sequences but did not contain significant levels of the smaller H-2L(d)-specific mRNA. The H-2L(d) antigen expressed on the surface of infected cells functioned as a target for cytolytic T cells specific for the H-2L(d) antigen.

Original languageEnglish (US)
Pages (from-to)1379-1384
Number of pages6
JournalMolecular and Cellular Biology
Volume5
Issue number6
StatePublished - 1985

Fingerprint

Histocompatibility Antigen H-2D
Retroviridae
Genome
Genes
RNA Splice Sites
Histocompatibility Antigens
Neomycin
Viral RNA
Transfection
Plasmids
Anti-Bacterial Agents
Viruses
T-Lymphocytes
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

H-2L(d) antigen encoded by a recombinant retrovirus genome is expressed on the surface of infected cells. / Weis, J. H.; Reiss, Carol; Mulligan, R. C.

In: Molecular and Cellular Biology, Vol. 5, No. 6, 1985, p. 1379-1384.

Research output: Contribution to journalArticle

@article{782be38ce08e4432a4ecfdc18aa70665,
title = "H-2L(d) antigen encoded by a recombinant retrovirus genome is expressed on the surface of infected cells",
abstract = "A recombinant murine retrovirus was constructed which contains, within its genome, a truncated version of the gene encoding the murine H-2L(d) major histocompatibility antigen. The H-2L(d) gene, which was inserted 3' of the env splice acceptor site in the recombinant retrovirus MSV-neo, lacked the 5' promoter and TATA sequences and the 3' transcription termination and polyadenylate addition sites of the normal H-2L(d) gene. Transfection of the MSV-neo/H-2L(d) plasmid (pLTV-11) into Y-2 cells resulted in the production of the transmissible recombinant retrovirus LTV-11. Cells infected with LTV-11 virus were resistant to the eucaryotic antibiotic G418 and expressed H-2L(d) on the cell surface. These infected cells contained a viral RNA species which possessed both the H-2L(d) and the neomycin resistance gene sequences but did not contain significant levels of the smaller H-2L(d)-specific mRNA. The H-2L(d) antigen expressed on the surface of infected cells functioned as a target for cytolytic T cells specific for the H-2L(d) antigen.",
author = "Weis, {J. H.} and Carol Reiss and Mulligan, {R. C.}",
year = "1985",
language = "English (US)",
volume = "5",
pages = "1379--1384",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - H-2L(d) antigen encoded by a recombinant retrovirus genome is expressed on the surface of infected cells

AU - Weis, J. H.

AU - Reiss, Carol

AU - Mulligan, R. C.

PY - 1985

Y1 - 1985

N2 - A recombinant murine retrovirus was constructed which contains, within its genome, a truncated version of the gene encoding the murine H-2L(d) major histocompatibility antigen. The H-2L(d) gene, which was inserted 3' of the env splice acceptor site in the recombinant retrovirus MSV-neo, lacked the 5' promoter and TATA sequences and the 3' transcription termination and polyadenylate addition sites of the normal H-2L(d) gene. Transfection of the MSV-neo/H-2L(d) plasmid (pLTV-11) into Y-2 cells resulted in the production of the transmissible recombinant retrovirus LTV-11. Cells infected with LTV-11 virus were resistant to the eucaryotic antibiotic G418 and expressed H-2L(d) on the cell surface. These infected cells contained a viral RNA species which possessed both the H-2L(d) and the neomycin resistance gene sequences but did not contain significant levels of the smaller H-2L(d)-specific mRNA. The H-2L(d) antigen expressed on the surface of infected cells functioned as a target for cytolytic T cells specific for the H-2L(d) antigen.

AB - A recombinant murine retrovirus was constructed which contains, within its genome, a truncated version of the gene encoding the murine H-2L(d) major histocompatibility antigen. The H-2L(d) gene, which was inserted 3' of the env splice acceptor site in the recombinant retrovirus MSV-neo, lacked the 5' promoter and TATA sequences and the 3' transcription termination and polyadenylate addition sites of the normal H-2L(d) gene. Transfection of the MSV-neo/H-2L(d) plasmid (pLTV-11) into Y-2 cells resulted in the production of the transmissible recombinant retrovirus LTV-11. Cells infected with LTV-11 virus were resistant to the eucaryotic antibiotic G418 and expressed H-2L(d) on the cell surface. These infected cells contained a viral RNA species which possessed both the H-2L(d) and the neomycin resistance gene sequences but did not contain significant levels of the smaller H-2L(d)-specific mRNA. The H-2L(d) antigen expressed on the surface of infected cells functioned as a target for cytolytic T cells specific for the H-2L(d) antigen.

UR - http://www.scopus.com/inward/record.url?scp=0021891332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021891332&partnerID=8YFLogxK

M3 - Article

VL - 5

SP - 1379

EP - 1384

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 6

ER -