Growth hormone (GH) stimulates insulin-like growth factor-I (IGF-I) and IGF-binding protein (IGFBP)-2 gene expression in spleens of juvenile rats

Shoshana Yakar, H. Domene, R. Meidan, F. Cassorla, I. Gilad, I. Koch, Z. Laron, C. T. Roberts, D. LeRoith, R. Eshet

Research output: Contribution to journalArticle

Abstract

Growth and development of the spleen involves the growth hormone (GH)/insulin-like growth factor-I IGF-I) axis. To evaluate the molecular mechanism of these effects we studied the effect of hypophysectomy (Hx) and GH replacement therapy on the expression of IGF-I, the IGF-I receptor and IGF-binding protein-2 (IGFBP-2) in juvenile rats. Hx resulted in a 30% reduction in body weight. GH replacement therapy for seven days partially prevented these effects. IGF-I mRNA levels were reduced 30% by Hx, IGFBP-2 mRNA levels fell 50% whereas IGF-I receptor mRNA levels were unaffected. GH therapy prevented the reduction in IGF-I and IGFBP-2 mRNA levels. These results suggest that the GH effect on splenic growth and development is via local (paracrine) IGF-I expression, in addition to any effect by circulating (endocrine) IGF-I.

Original languageEnglish (US)
Pages (from-to)363-366
Number of pages4
JournalHormone and Metabolic Research
Volume26
Issue number8
Publication statusPublished - 1994

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Keywords

  • Gene expression
  • Growth hormone
  • IGF-I
  • Spleen

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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