Growth hormone (GH) stimulates insulin-like growth factor-I (IGF-I) and IGF-binding protein (IGFBP)-2 gene expression in spleens of juvenile rats

Shoshana Yakar, H. Domene, R. Meidan, F. Cassorla, I. Gilad, I. Koch, Z. Laron, C. T. Roberts, D. LeRoith, R. Eshet

Research output: Contribution to journalArticle

Abstract

Growth and development of the spleen involves the growth hormone (GH)/insulin-like growth factor-I IGF-I) axis. To evaluate the molecular mechanism of these effects we studied the effect of hypophysectomy (Hx) and GH replacement therapy on the expression of IGF-I, the IGF-I receptor and IGF-binding protein-2 (IGFBP-2) in juvenile rats. Hx resulted in a 30% reduction in body weight. GH replacement therapy for seven days partially prevented these effects. IGF-I mRNA levels were reduced 30% by Hx, IGFBP-2 mRNA levels fell 50% whereas IGF-I receptor mRNA levels were unaffected. GH therapy prevented the reduction in IGF-I and IGFBP-2 mRNA levels. These results suggest that the GH effect on splenic growth and development is via local (paracrine) IGF-I expression, in addition to any effect by circulating (endocrine) IGF-I.

Original languageEnglish (US)
Pages (from-to)363-366
Number of pages4
JournalHormone and Metabolic Research
Volume26
Issue number8
StatePublished - 1994

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Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor I
Gene expression
Growth Hormone
Rats
Spleen
Gene Expression
IGF Type 1 Receptor
Messenger RNA
Hormone Replacement Therapy
Growth and Development
Hypophysectomy
Body Weight

Keywords

  • Gene expression
  • Growth hormone
  • IGF-I
  • Spleen

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Growth hormone (GH) stimulates insulin-like growth factor-I (IGF-I) and IGF-binding protein (IGFBP)-2 gene expression in spleens of juvenile rats. / Yakar, Shoshana; Domene, H.; Meidan, R.; Cassorla, F.; Gilad, I.; Koch, I.; Laron, Z.; Roberts, C. T.; LeRoith, D.; Eshet, R.

In: Hormone and Metabolic Research, Vol. 26, No. 8, 1994, p. 363-366.

Research output: Contribution to journalArticle

Yakar, S, Domene, H, Meidan, R, Cassorla, F, Gilad, I, Koch, I, Laron, Z, Roberts, CT, LeRoith, D & Eshet, R 1994, 'Growth hormone (GH) stimulates insulin-like growth factor-I (IGF-I) and IGF-binding protein (IGFBP)-2 gene expression in spleens of juvenile rats', Hormone and Metabolic Research, vol. 26, no. 8, pp. 363-366.
Yakar, Shoshana ; Domene, H. ; Meidan, R. ; Cassorla, F. ; Gilad, I. ; Koch, I. ; Laron, Z. ; Roberts, C. T. ; LeRoith, D. ; Eshet, R. / Growth hormone (GH) stimulates insulin-like growth factor-I (IGF-I) and IGF-binding protein (IGFBP)-2 gene expression in spleens of juvenile rats. In: Hormone and Metabolic Research. 1994 ; Vol. 26, No. 8. pp. 363-366.
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abstract = "Growth and development of the spleen involves the growth hormone (GH)/insulin-like growth factor-I IGF-I) axis. To evaluate the molecular mechanism of these effects we studied the effect of hypophysectomy (Hx) and GH replacement therapy on the expression of IGF-I, the IGF-I receptor and IGF-binding protein-2 (IGFBP-2) in juvenile rats. Hx resulted in a 30{\%} reduction in body weight. GH replacement therapy for seven days partially prevented these effects. IGF-I mRNA levels were reduced 30{\%} by Hx, IGFBP-2 mRNA levels fell 50{\%} whereas IGF-I receptor mRNA levels were unaffected. GH therapy prevented the reduction in IGF-I and IGFBP-2 mRNA levels. These results suggest that the GH effect on splenic growth and development is via local (paracrine) IGF-I expression, in addition to any effect by circulating (endocrine) IGF-I.",
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AU - Yakar, Shoshana

AU - Domene, H.

AU - Meidan, R.

AU - Cassorla, F.

AU - Gilad, I.

AU - Koch, I.

AU - Laron, Z.

AU - Roberts, C. T.

AU - LeRoith, D.

AU - Eshet, R.

PY - 1994

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N2 - Growth and development of the spleen involves the growth hormone (GH)/insulin-like growth factor-I IGF-I) axis. To evaluate the molecular mechanism of these effects we studied the effect of hypophysectomy (Hx) and GH replacement therapy on the expression of IGF-I, the IGF-I receptor and IGF-binding protein-2 (IGFBP-2) in juvenile rats. Hx resulted in a 30% reduction in body weight. GH replacement therapy for seven days partially prevented these effects. IGF-I mRNA levels were reduced 30% by Hx, IGFBP-2 mRNA levels fell 50% whereas IGF-I receptor mRNA levels were unaffected. GH therapy prevented the reduction in IGF-I and IGFBP-2 mRNA levels. These results suggest that the GH effect on splenic growth and development is via local (paracrine) IGF-I expression, in addition to any effect by circulating (endocrine) IGF-I.

AB - Growth and development of the spleen involves the growth hormone (GH)/insulin-like growth factor-I IGF-I) axis. To evaluate the molecular mechanism of these effects we studied the effect of hypophysectomy (Hx) and GH replacement therapy on the expression of IGF-I, the IGF-I receptor and IGF-binding protein-2 (IGFBP-2) in juvenile rats. Hx resulted in a 30% reduction in body weight. GH replacement therapy for seven days partially prevented these effects. IGF-I mRNA levels were reduced 30% by Hx, IGFBP-2 mRNA levels fell 50% whereas IGF-I receptor mRNA levels were unaffected. GH therapy prevented the reduction in IGF-I and IGFBP-2 mRNA levels. These results suggest that the GH effect on splenic growth and development is via local (paracrine) IGF-I expression, in addition to any effect by circulating (endocrine) IGF-I.

KW - Gene expression

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KW - IGF-I

KW - Spleen

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