Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid

Anu Singh, Gaurav Sinsinbar, Meenakshi Choudhary, Veeresh Kumar, Renu Pasricha, H. N. Verma, Surinder P. Singh, Kavita Arora

Research output: Contribution to journalArticle

Abstract

Graphene oxide (GO)-Chitosan (CHI) nano-composite is employed for the development of DNA based electrochemical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi specific 5′-amine labeled single stranded (ss) DNA probe on GO-CHI/ITO via glutaraldehyde. Differential pulse voltammetry (DPV) studies revealed good specificity and ability of ssDNA/GO-CHI/ITO biosensor to distinguish complementary, non-complementary and one base mismatch sequences. The ssDNA/GO-CHI/ITO biosensor showed detection range of 10 fM to 50 nM and LOD 10 fM within 60 s hybridization times for complementary sequence. Further, ssDNA/GO-CHI/ITO bioelectrode is able to detect complementary target present in serum samples with LOD of 100 fM at 25 C. The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO).

Original languageEnglish (US)
Pages (from-to)675-684
Number of pages10
JournalSensors and Actuators, B: Chemical
Volume185
DOIs
StatePublished - Jul 1 2013

Fingerprint

typhoid
Graphite
Chitosan
bioinstrumentation
Biosensors
Oxides
Graphene
ITO (semiconductors)
Nanocomposites
nanocomposites
graphene
DNA
deoxyribonucleic acid
oxides
immobilization
salmonella
Salmonella
Single-Stranded DNA
DNA Probes
Glutaral

Keywords

  • Chitosan
  • DNA
  • Graphene oxide
  • Nano composite
  • Salmonella
  • Typhoid

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid. / Singh, Anu; Sinsinbar, Gaurav; Choudhary, Meenakshi; Kumar, Veeresh; Pasricha, Renu; Verma, H. N.; Singh, Surinder P.; Arora, Kavita.

In: Sensors and Actuators, B: Chemical, Vol. 185, 01.07.2013, p. 675-684.

Research output: Contribution to journalArticle

Singh, Anu ; Sinsinbar, Gaurav ; Choudhary, Meenakshi ; Kumar, Veeresh ; Pasricha, Renu ; Verma, H. N. ; Singh, Surinder P. ; Arora, Kavita. / Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid. In: Sensors and Actuators, B: Chemical. 2013 ; Vol. 185. pp. 675-684.
@article{09847f6eff9e41c6b7be7c2def15db88,
title = "Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid",
abstract = "Graphene oxide (GO)-Chitosan (CHI) nano-composite is employed for the development of DNA based electrochemical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi specific 5′-amine labeled single stranded (ss) DNA probe on GO-CHI/ITO via glutaraldehyde. Differential pulse voltammetry (DPV) studies revealed good specificity and ability of ssDNA/GO-CHI/ITO biosensor to distinguish complementary, non-complementary and one base mismatch sequences. The ssDNA/GO-CHI/ITO biosensor showed detection range of 10 fM to 50 nM and LOD 10 fM within 60 s hybridization times for complementary sequence. Further, ssDNA/GO-CHI/ITO bioelectrode is able to detect complementary target present in serum samples with LOD of 100 fM at 25 C. The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO).",
keywords = "Chitosan, DNA, Graphene oxide, Nano composite, Salmonella, Typhoid",
author = "Anu Singh and Gaurav Sinsinbar and Meenakshi Choudhary and Veeresh Kumar and Renu Pasricha and Verma, {H. N.} and Singh, {Surinder P.} and Kavita Arora",
year = "2013",
month = "7",
day = "1",
doi = "10.1016/j.snb.2013.05.014",
language = "English (US)",
volume = "185",
pages = "675--684",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

TY - JOUR

T1 - Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid

AU - Singh, Anu

AU - Sinsinbar, Gaurav

AU - Choudhary, Meenakshi

AU - Kumar, Veeresh

AU - Pasricha, Renu

AU - Verma, H. N.

AU - Singh, Surinder P.

AU - Arora, Kavita

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Graphene oxide (GO)-Chitosan (CHI) nano-composite is employed for the development of DNA based electrochemical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi specific 5′-amine labeled single stranded (ss) DNA probe on GO-CHI/ITO via glutaraldehyde. Differential pulse voltammetry (DPV) studies revealed good specificity and ability of ssDNA/GO-CHI/ITO biosensor to distinguish complementary, non-complementary and one base mismatch sequences. The ssDNA/GO-CHI/ITO biosensor showed detection range of 10 fM to 50 nM and LOD 10 fM within 60 s hybridization times for complementary sequence. Further, ssDNA/GO-CHI/ITO bioelectrode is able to detect complementary target present in serum samples with LOD of 100 fM at 25 C. The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO).

AB - Graphene oxide (GO)-Chitosan (CHI) nano-composite is employed for the development of DNA based electrochemical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi specific 5′-amine labeled single stranded (ss) DNA probe on GO-CHI/ITO via glutaraldehyde. Differential pulse voltammetry (DPV) studies revealed good specificity and ability of ssDNA/GO-CHI/ITO biosensor to distinguish complementary, non-complementary and one base mismatch sequences. The ssDNA/GO-CHI/ITO biosensor showed detection range of 10 fM to 50 nM and LOD 10 fM within 60 s hybridization times for complementary sequence. Further, ssDNA/GO-CHI/ITO bioelectrode is able to detect complementary target present in serum samples with LOD of 100 fM at 25 C. The excellent performance of biosensor is attributed to large surface-to-volume ratio and good electrochemical activity of graphene oxide, and good biocompatibility of chitosan, which enhances the DNA immobilization and facilitate electron transfer between DNA and electrode surface (ITO).

KW - Chitosan

KW - DNA

KW - Graphene oxide

KW - Nano composite

KW - Salmonella

KW - Typhoid

UR - http://www.scopus.com/inward/record.url?scp=84879328992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879328992&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2013.05.014

DO - 10.1016/j.snb.2013.05.014

M3 - Article

VL - 185

SP - 675

EP - 684

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

ER -