Glycosylation Fosters Interactions between Model Sea Urchin Spicule Matrix Proteins. Implications for Embryonic Spiculogenesis and Biomineralization

Gaurav Jain, Martin Pendola, Eleni Koutsoumpeli, Steven Johnson, John Evans

Research output: Contribution to journalArticle

Abstract

The formation of embryonic mineralized skeletal elements (spicules) in the sea urchin requires the participation of proteins, many of which may interact with one another and assist in the creation of an extracellular matrix wherein mineral formation takes place. To probe this, we created a sea urchin spicule recombinant model protein pair system wherein we tested the interactions between two major spicule proteins, SpSM50 and the glycoprotein, SpSM30B/C. Both proteins are strong hydrogelators that manipulate early and later events in mineral formation. We discovered that the anionic glycan moieties of SpSM30B/C are required for interaction with the SpSM50 protein and that these interactions are Ca(II)-independent. In addition, when these proteins form a complex, they create hybrid hydrogel particles that are physically distinct from their individual counterparts. Thus, glycan-mediated interactions play an important role in in vitro spicule protein assembly and most likely within the spicule itself.

Original languageEnglish (US)
Pages (from-to)3032-3035
Number of pages4
JournalBiochemistry
Volume57
Issue number21
DOIs
StatePublished - May 29 2018

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Glycosylation
Biomineralization
Sea Urchins
Proteins
Minerals
Polysaccharides
Hydrogel
Recombinant Proteins
Extracellular Matrix

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glycosylation Fosters Interactions between Model Sea Urchin Spicule Matrix Proteins. Implications for Embryonic Spiculogenesis and Biomineralization. / Jain, Gaurav; Pendola, Martin; Koutsoumpeli, Eleni; Johnson, Steven; Evans, John.

In: Biochemistry, Vol. 57, No. 21, 29.05.2018, p. 3032-3035.

Research output: Contribution to journalArticle

Jain, Gaurav ; Pendola, Martin ; Koutsoumpeli, Eleni ; Johnson, Steven ; Evans, John. / Glycosylation Fosters Interactions between Model Sea Urchin Spicule Matrix Proteins. Implications for Embryonic Spiculogenesis and Biomineralization. In: Biochemistry. 2018 ; Vol. 57, No. 21. pp. 3032-3035.
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