Glycine mediated alterations in intracellular pH

Joshua S. Green, Vibhakar C. Kotak, Dan Sanes

Research output: Contribution to journalArticle

Abstract

Glycinergic transmission shapes the coding properties of the lateral superior olivary nucleus (LSO). We investigated intracellular pH responses in the LSO to glycine using BCECF-AM in brain slices. With extracellular bicarbonate, glycine produced an alkalinization followed by an acidification while, in the nominal absence of bicarbonate, glycine produced acidifications. Separately, in whole-cell recordings from LSO neurons, glycine caused hyperpolarization followed by long-lasting depolarization. While the bicarbonate-dependent intracellular alkalinization could be related to chloride/bicarbonate exchange, bicarbonate-independent acidification may be triggered by depolarization.

Original languageEnglish (US)
Pages (from-to)122-127
Number of pages6
JournalBrain Research
Volume989
Issue number1
DOIs
StatePublished - 2003

Fingerprint

Bicarbonates
Glycine
Patch-Clamp Techniques
Chlorides
Neurons
Brain
Superior Olivary Complex

Keywords

  • Auditory
  • Glycine
  • Inhibition
  • pH

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Glycine mediated alterations in intracellular pH. / Green, Joshua S.; Kotak, Vibhakar C.; Sanes, Dan.

In: Brain Research, Vol. 989, No. 1, 2003, p. 122-127.

Research output: Contribution to journalArticle

Green, Joshua S. ; Kotak, Vibhakar C. ; Sanes, Dan. / Glycine mediated alterations in intracellular pH. In: Brain Research. 2003 ; Vol. 989, No. 1. pp. 122-127.
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