Glutamate spillover promotes the generation of NMDA spikes

Jason R. Chalifoux, Adam Carter

Research output: Contribution to journalArticle

Abstract

NMDA spikes are prominent in the basal dendrites of cortical pyramidal neurons and greatly expand their ability to integrate synaptic inputs. Calcium (Ca) signals during these spikes are important for synaptic plasticity and fundamentally depend on activation of NMDA receptors. However, the factors that shape the activation of these receptors and the initiation of NMDA spikes remain unclear. Here we examine the properties of NMDA spikes in the basal dendrites of layer 5 pyramidal neurons in the mouse prefrontal cortex. Using two-photon imaging, we demonstrate thatNMDAspikes evoke large Ca signals in both postsynaptic spines and nearby dendrites.Wefind that the dendrite Ca signals depend onNMDAandAMPAreceptors but not sodium (Na) or Ca channels. Using voltage-clamp recordings, we show that activation of dendrite NMDA receptors is enhanced by concerted synaptic activity. Blocking glutamate reuptake further increases activation of these receptors and promotes the initiation of NMDA spikes. We conclude that glutamate spillover and recruitment of extrasynaptic receptors contribute to the initiation ofNMDAspikes. These results have important implications for how synaptic activity generates both electrical and biochemical signals in dendrites and spines.

Original languageEnglish (US)
Pages (from-to)16435-16446
Number of pages12
JournalJournal of Neuroscience
Volume31
Issue number45
DOIs
StatePublished - Nov 9 2011

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N-Methylaspartate
Dendrites
Glutamic Acid
N-Methyl-D-Aspartate Receptors
Pyramidal Cells
Calcium
Spine
Neuronal Plasticity
Sodium Channels
Calcium Channels
Prefrontal Cortex
Photons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Glutamate spillover promotes the generation of NMDA spikes. / Chalifoux, Jason R.; Carter, Adam.

In: Journal of Neuroscience, Vol. 31, No. 45, 09.11.2011, p. 16435-16446.

Research output: Contribution to journalArticle

Chalifoux, Jason R. ; Carter, Adam. / Glutamate spillover promotes the generation of NMDA spikes. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 45. pp. 16435-16446.
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