Global studies of cell type-specific gene expression in plants

David W. Galbraith, Kenneth Birnbaum

Research output: Contribution to journalArticle

Abstract

Technological advances in expression profiling and in the ability to collect minute quantities of tissues have come together to allow a growing number of global transcriptional studies at the cell level in plants. Microarray technology, with a choice of cDNA or oligo-based slides, is now well established, with commercial full-genome platforms for rice and Arabidopsis and extensive expressed sequence tag (EST)-based designs for many other species. Microdissection and cell sorting are two established methodologies that have been used in conjunction with microarrays to provide an early glimpse of the transcriptional landscape at the level of individual cell types. The results indicate that much of the transcriptome is compartmentalized. A minor but consistent percentage of transcripts appear to be unique to specific cell types. Functional analyses of cell-specific patterns of gene expression are providing important clues to cell-specific functions. The spatial dissection of the transcriptome has also yielded insights into the localized mediators of hormone inputs and promises to provide detail on cell-specific effects of microRNAs.

Original languageEnglish (US)
Pages (from-to)451-475
Number of pages25
JournalAnnual Review of Plant Biology
Volume57
DOIs
StatePublished - 2006

Fingerprint

Microarrays
Gene expression
Microdissection
Gene Expression
Dissection
gene expression
Expressed Sequence Tags
MicroRNAs
Sorting
Complementary DNA
Genes
Cells
Hormones
cells
Tissue
Transcriptome
transcriptome
microarray technology
expressed sequence tags
microRNA

Keywords

  • Cell identity
  • Cell-specific expression analysis
  • Differentiation
  • Microdissection

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Global studies of cell type-specific gene expression in plants. / Galbraith, David W.; Birnbaum, Kenneth.

In: Annual Review of Plant Biology, Vol. 57, 2006, p. 451-475.

Research output: Contribution to journalArticle

@article{f12e493ef3e548c19a0c9d7e0d2e7ab9,
title = "Global studies of cell type-specific gene expression in plants",
abstract = "Technological advances in expression profiling and in the ability to collect minute quantities of tissues have come together to allow a growing number of global transcriptional studies at the cell level in plants. Microarray technology, with a choice of cDNA or oligo-based slides, is now well established, with commercial full-genome platforms for rice and Arabidopsis and extensive expressed sequence tag (EST)-based designs for many other species. Microdissection and cell sorting are two established methodologies that have been used in conjunction with microarrays to provide an early glimpse of the transcriptional landscape at the level of individual cell types. The results indicate that much of the transcriptome is compartmentalized. A minor but consistent percentage of transcripts appear to be unique to specific cell types. Functional analyses of cell-specific patterns of gene expression are providing important clues to cell-specific functions. The spatial dissection of the transcriptome has also yielded insights into the localized mediators of hormone inputs and promises to provide detail on cell-specific effects of microRNAs.",
keywords = "Cell identity, Cell-specific expression analysis, Differentiation, Microdissection",
author = "Galbraith, {David W.} and Kenneth Birnbaum",
year = "2006",
doi = "10.1146/annurev.arplant.57.032905.105302",
language = "English (US)",
volume = "57",
pages = "451--475",
journal = "Annual Review of Plant Biology",
issn = "1543-5008",
publisher = "Annual Reviews Inc.",

}

TY - JOUR

T1 - Global studies of cell type-specific gene expression in plants

AU - Galbraith, David W.

AU - Birnbaum, Kenneth

PY - 2006

Y1 - 2006

N2 - Technological advances in expression profiling and in the ability to collect minute quantities of tissues have come together to allow a growing number of global transcriptional studies at the cell level in plants. Microarray technology, with a choice of cDNA or oligo-based slides, is now well established, with commercial full-genome platforms for rice and Arabidopsis and extensive expressed sequence tag (EST)-based designs for many other species. Microdissection and cell sorting are two established methodologies that have been used in conjunction with microarrays to provide an early glimpse of the transcriptional landscape at the level of individual cell types. The results indicate that much of the transcriptome is compartmentalized. A minor but consistent percentage of transcripts appear to be unique to specific cell types. Functional analyses of cell-specific patterns of gene expression are providing important clues to cell-specific functions. The spatial dissection of the transcriptome has also yielded insights into the localized mediators of hormone inputs and promises to provide detail on cell-specific effects of microRNAs.

AB - Technological advances in expression profiling and in the ability to collect minute quantities of tissues have come together to allow a growing number of global transcriptional studies at the cell level in plants. Microarray technology, with a choice of cDNA or oligo-based slides, is now well established, with commercial full-genome platforms for rice and Arabidopsis and extensive expressed sequence tag (EST)-based designs for many other species. Microdissection and cell sorting are two established methodologies that have been used in conjunction with microarrays to provide an early glimpse of the transcriptional landscape at the level of individual cell types. The results indicate that much of the transcriptome is compartmentalized. A minor but consistent percentage of transcripts appear to be unique to specific cell types. Functional analyses of cell-specific patterns of gene expression are providing important clues to cell-specific functions. The spatial dissection of the transcriptome has also yielded insights into the localized mediators of hormone inputs and promises to provide detail on cell-specific effects of microRNAs.

KW - Cell identity

KW - Cell-specific expression analysis

KW - Differentiation

KW - Microdissection

UR - http://www.scopus.com/inward/record.url?scp=33745965470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745965470&partnerID=8YFLogxK

U2 - 10.1146/annurev.arplant.57.032905.105302

DO - 10.1146/annurev.arplant.57.032905.105302

M3 - Article

C2 - 16669770

AN - SCOPUS:33745965470

VL - 57

SP - 451

EP - 475

JO - Annual Review of Plant Biology

JF - Annual Review of Plant Biology

SN - 1543-5008

ER -