Geometrical versus random β-TCP scaffolds

Exploring the effects on Schwann cell growth and behavior

Lauren Sweet, Yunqing Kang, Christopher Czisch, Lukasz Witek, Yang Shi, Jim Smay, Giles W. Plant, Yunzhi Yang

Research output: Contribution to journalArticle

Abstract

Numerous studies have demonstrated that Schwann cells (SCs) play a role in nerve regeneration; however, their role in innervating a bioceramic scaffold for potential application in bone regeneration is still unknown. Here we report the cell growth and functional behavior of SCs on β-tricalcium phosphate (β-TCP) scaffolds arranged in 3D printed-lattice (P-β-TCP) and randomly-porous, template-casted (N-β-TCP) structures. Our results indicate that SCs proliferated well and expressed the phenotypic markers p75LNGFR and the S100-β subunit of SCs as well as displayed growth morphology on both scaffolds, but SCs showed spindle-shaped morphology with a significant degree of SCs alignment on the P-β-TCP scaffolds, seen to a lesser degree in the N-β-TCP scaffold. The gene expressions of nerve growth factor (β-ngf), neutrophin-3 (nt-3), platelet-derived growth factor (pdgf-bb), and vascular endothelial growth factor (vegf-a) were higher at day 7 than at day 14. While no significant differences in protein secretion were measured between these last two time points, the scaffolds promoted the protein secretion at day 3 compared to that on the cell culture plates. These results together imply that the β-TCP scaffolds can support SC cell growth and that the 3D-printed scaffold appeared to significantly promote the alignment of SCs along the struts. Further studies are needed to investigate the early and late stage relationship between gene expression and protein secretion of SCs on the scaffolds with refined characteristics, thus better exploring the potential of SCs to support vascularization and innervation in synthetic bone grafts.

Original languageEnglish (US)
Article numbere013982
JournalPLoS One
Volume10
Issue number10
DOIs
StatePublished - Oct 7 2015

Fingerprint

Schwann cells
Schwann Cells
Cell growth
Scaffolds (biology)
Scaffolds
cell growth
Growth
protein secretion
Gene expression
Bone
Cells
Bioceramics
Proteins
Struts
Platelet-Derived Growth Factor
Nerve Growth Factor
bones
scaffolding proteins
Cell culture
Grafts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Geometrical versus random β-TCP scaffolds : Exploring the effects on Schwann cell growth and behavior. / Sweet, Lauren; Kang, Yunqing; Czisch, Christopher; Witek, Lukasz; Shi, Yang; Smay, Jim; Plant, Giles W.; Yang, Yunzhi.

In: PLoS One, Vol. 10, No. 10, e013982, 07.10.2015.

Research output: Contribution to journalArticle

Sweet, Lauren ; Kang, Yunqing ; Czisch, Christopher ; Witek, Lukasz ; Shi, Yang ; Smay, Jim ; Plant, Giles W. ; Yang, Yunzhi. / Geometrical versus random β-TCP scaffolds : Exploring the effects on Schwann cell growth and behavior. In: PLoS One. 2015 ; Vol. 10, No. 10.
@article{751ffd02002243c68204966a80fe714c,
title = "Geometrical versus random β-TCP scaffolds: Exploring the effects on Schwann cell growth and behavior",
abstract = "Numerous studies have demonstrated that Schwann cells (SCs) play a role in nerve regeneration; however, their role in innervating a bioceramic scaffold for potential application in bone regeneration is still unknown. Here we report the cell growth and functional behavior of SCs on β-tricalcium phosphate (β-TCP) scaffolds arranged in 3D printed-lattice (P-β-TCP) and randomly-porous, template-casted (N-β-TCP) structures. Our results indicate that SCs proliferated well and expressed the phenotypic markers p75LNGFR and the S100-β subunit of SCs as well as displayed growth morphology on both scaffolds, but SCs showed spindle-shaped morphology with a significant degree of SCs alignment on the P-β-TCP scaffolds, seen to a lesser degree in the N-β-TCP scaffold. The gene expressions of nerve growth factor (β-ngf), neutrophin-3 (nt-3), platelet-derived growth factor (pdgf-bb), and vascular endothelial growth factor (vegf-a) were higher at day 7 than at day 14. While no significant differences in protein secretion were measured between these last two time points, the scaffolds promoted the protein secretion at day 3 compared to that on the cell culture plates. These results together imply that the β-TCP scaffolds can support SC cell growth and that the 3D-printed scaffold appeared to significantly promote the alignment of SCs along the struts. Further studies are needed to investigate the early and late stage relationship between gene expression and protein secretion of SCs on the scaffolds with refined characteristics, thus better exploring the potential of SCs to support vascularization and innervation in synthetic bone grafts.",
author = "Lauren Sweet and Yunqing Kang and Christopher Czisch and Lukasz Witek and Yang Shi and Jim Smay and Plant, {Giles W.} and Yunzhi Yang",
year = "2015",
month = "10",
day = "7",
doi = "10.1371/journal.pone.0139820",
language = "English (US)",
volume = "10",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

TY - JOUR

T1 - Geometrical versus random β-TCP scaffolds

T2 - Exploring the effects on Schwann cell growth and behavior

AU - Sweet, Lauren

AU - Kang, Yunqing

AU - Czisch, Christopher

AU - Witek, Lukasz

AU - Shi, Yang

AU - Smay, Jim

AU - Plant, Giles W.

AU - Yang, Yunzhi

PY - 2015/10/7

Y1 - 2015/10/7

N2 - Numerous studies have demonstrated that Schwann cells (SCs) play a role in nerve regeneration; however, their role in innervating a bioceramic scaffold for potential application in bone regeneration is still unknown. Here we report the cell growth and functional behavior of SCs on β-tricalcium phosphate (β-TCP) scaffolds arranged in 3D printed-lattice (P-β-TCP) and randomly-porous, template-casted (N-β-TCP) structures. Our results indicate that SCs proliferated well and expressed the phenotypic markers p75LNGFR and the S100-β subunit of SCs as well as displayed growth morphology on both scaffolds, but SCs showed spindle-shaped morphology with a significant degree of SCs alignment on the P-β-TCP scaffolds, seen to a lesser degree in the N-β-TCP scaffold. The gene expressions of nerve growth factor (β-ngf), neutrophin-3 (nt-3), platelet-derived growth factor (pdgf-bb), and vascular endothelial growth factor (vegf-a) were higher at day 7 than at day 14. While no significant differences in protein secretion were measured between these last two time points, the scaffolds promoted the protein secretion at day 3 compared to that on the cell culture plates. These results together imply that the β-TCP scaffolds can support SC cell growth and that the 3D-printed scaffold appeared to significantly promote the alignment of SCs along the struts. Further studies are needed to investigate the early and late stage relationship between gene expression and protein secretion of SCs on the scaffolds with refined characteristics, thus better exploring the potential of SCs to support vascularization and innervation in synthetic bone grafts.

AB - Numerous studies have demonstrated that Schwann cells (SCs) play a role in nerve regeneration; however, their role in innervating a bioceramic scaffold for potential application in bone regeneration is still unknown. Here we report the cell growth and functional behavior of SCs on β-tricalcium phosphate (β-TCP) scaffolds arranged in 3D printed-lattice (P-β-TCP) and randomly-porous, template-casted (N-β-TCP) structures. Our results indicate that SCs proliferated well and expressed the phenotypic markers p75LNGFR and the S100-β subunit of SCs as well as displayed growth morphology on both scaffolds, but SCs showed spindle-shaped morphology with a significant degree of SCs alignment on the P-β-TCP scaffolds, seen to a lesser degree in the N-β-TCP scaffold. The gene expressions of nerve growth factor (β-ngf), neutrophin-3 (nt-3), platelet-derived growth factor (pdgf-bb), and vascular endothelial growth factor (vegf-a) were higher at day 7 than at day 14. While no significant differences in protein secretion were measured between these last two time points, the scaffolds promoted the protein secretion at day 3 compared to that on the cell culture plates. These results together imply that the β-TCP scaffolds can support SC cell growth and that the 3D-printed scaffold appeared to significantly promote the alignment of SCs along the struts. Further studies are needed to investigate the early and late stage relationship between gene expression and protein secretion of SCs on the scaffolds with refined characteristics, thus better exploring the potential of SCs to support vascularization and innervation in synthetic bone grafts.

UR - http://www.scopus.com/inward/record.url?scp=84956623176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84956623176&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0139820

DO - 10.1371/journal.pone.0139820

M3 - Article

VL - 10

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 10

M1 - e013982

ER -