Genomic organization of the 8p11∼p12 amplicon in three breast cancer cell lines

Zeng Quan Yang, Donna Albertson, Stephen P. Ethier

Research output: Contribution to journalArticle

Abstract

Amplification of chromosomal regions leads to an increase of DNA copy number and expression of oncogenes in human breast cancer (HBC). Amplification of the 8p11∼p12 region occurs in 10-15% of primary, uncultured HBCs. In our panel of 11 breast cancer cells, three cell lines, SUM-44, SUM-52, and SUM-225, have overlapping amplicons in the 8p11∼p12 region. To characterize genome structure of the amplified regions, we performed fluorescence in situ hybridization using 8p11∼p12 BAC clones in the 3 cell lines. The results revealed that the 8p11∼p12 amplicon has a highly complex structure and that FGFR1 is not in the common core-amplified domain in 3 breast cancer cell lines with the amplicon. These 3 cell lines provide good models for genetic and functional studies of candidate oncogenes of the 8p11∼p12 region.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalCancer Genetics and Cytogenetics
Volume155
Issue number1
DOIs
StatePublished - Nov 1 2004

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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