Genomic and Expression Analysis of the 8p11-12 Amplicon in Human Breast Cancer Cell Lines

Michael E. Ray, Zeng Quan Yang, Donna Albertson, Celina G. Kleer, Joseph G. Washburn, Jill A. Macoska, Stephen P. Ethier

Research output: Contribution to journalArticle

Abstract

Gene amplification is an important mechanism of oncogene activation in breast and other cancers. Characterization of amplified regions of the genome in breast cancer has led to the identification of important oncogenes including erbB-2/HER-2, C-MYC, and fibroblast growth factor receptor (FGFR) 2. Chromosome 8p11-p12 is amplified in 10-15% of human breast cancers. The putative oncogene FGFR1 localizes to this region; however, we show evidence that FGFR inhibition fails to slow growth of three breast cancer cell lines with 8p11-p12 amplification. We present a detailed analysis of this amplicon in three human breast cancer cell lines using comparative genomic hybridization, traditional Southern and Northern analysis, and chromosome 8 cDNA microarray expression profiling. This study has identified new candidate oncogenes within the 8p11-p12 region, supporting the hypothesis that genes other than FGFR1 may contribute to oncogenesis in breast cancers with proximal 8p amplification.

Original languageEnglish (US)
Pages (from-to)40-47
Number of pages8
JournalCancer Research
Volume64
Issue number1
DOIs
StatePublished - Jan 1 2004

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Breast Neoplasms
Oncogenes
Cell Line
Receptor, Fibroblast Growth Factor, Type 2
Fibroblast Growth Factor Receptors
Chromosomes, Human, Pair 8
Comparative Genomic Hybridization
Gene Amplification
Oligonucleotide Array Sequence Analysis
Carcinogenesis
Chromosomes
Genome
Growth
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ray, M. E., Yang, Z. Q., Albertson, D., Kleer, C. G., Washburn, J. G., Macoska, J. A., & Ethier, S. P. (2004). Genomic and Expression Analysis of the 8p11-12 Amplicon in Human Breast Cancer Cell Lines. Cancer Research, 64(1), 40-47. https://doi.org/10.1158/0008-5472.CAN-03-1022

Genomic and Expression Analysis of the 8p11-12 Amplicon in Human Breast Cancer Cell Lines. / Ray, Michael E.; Yang, Zeng Quan; Albertson, Donna; Kleer, Celina G.; Washburn, Joseph G.; Macoska, Jill A.; Ethier, Stephen P.

In: Cancer Research, Vol. 64, No. 1, 01.01.2004, p. 40-47.

Research output: Contribution to journalArticle

Ray, Michael E. ; Yang, Zeng Quan ; Albertson, Donna ; Kleer, Celina G. ; Washburn, Joseph G. ; Macoska, Jill A. ; Ethier, Stephen P. / Genomic and Expression Analysis of the 8p11-12 Amplicon in Human Breast Cancer Cell Lines. In: Cancer Research. 2004 ; Vol. 64, No. 1. pp. 40-47.
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