Genomic analysis of the nitrate response using a nitrate reductase-null mutant of arabidopsis

Rongchen Wang, Rudolf Tischner, Rodrigo A. Gutiérrez, Maren Hoffman, Xiujuan Xing, Mingsheng Chen, Gloria Coruzzi, Nigel M. Crawford

Research output: Contribution to journalArticle

Abstract

A nitrate reductase (NR)-null mutant of Arabidopsis was constructed that had a deletion of the major NR gene NIA2 and an insertion in the NIA1 NR gene. This mutant had no detectable NR activity and could not use nitrate as the sole nitrogen source. Starch mobilization was not induced by nitrate in this mutant but was induced by ammonium, indicating that nitrate was not the signal for this process. Microarray analysis of gene expression revealed that 595 genes responded to nitrate (5 mM nitrate for 2 h) in both wild-type and mutant plants. This group of genes was overrepresented most significantly in the functional categories of energy, metabolism, and glycolysis and gluconeogenesis. Because the nitrate response of these genes was NR independent, nitrate and not a downstream metabolite served as the signal. The microarray analysis also revealed that shoots can be as responsive to nitrate as roots, yet there was substantial organ specificity to the nitrate response.

Original languageEnglish (US)
Pages (from-to)2512-2522
Number of pages11
JournalPlant Physiology
Volume136
Issue number1
DOIs
StatePublished - Sep 2004

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nitrate reductase
nitrates
Arabidopsis
genomics
mutants
genes
gluconeogenesis
glycolysis
ammonium nitrate
energy metabolism
starch
metabolites
gene expression
shoots
nitrogen

ASJC Scopus subject areas

  • Plant Science

Cite this

Wang, R., Tischner, R., Gutiérrez, R. A., Hoffman, M., Xing, X., Chen, M., ... Crawford, N. M. (2004). Genomic analysis of the nitrate response using a nitrate reductase-null mutant of arabidopsis. Plant Physiology, 136(1), 2512-2522. https://doi.org/10.1104/pp.104.044610

Genomic analysis of the nitrate response using a nitrate reductase-null mutant of arabidopsis. / Wang, Rongchen; Tischner, Rudolf; Gutiérrez, Rodrigo A.; Hoffman, Maren; Xing, Xiujuan; Chen, Mingsheng; Coruzzi, Gloria; Crawford, Nigel M.

In: Plant Physiology, Vol. 136, No. 1, 09.2004, p. 2512-2522.

Research output: Contribution to journalArticle

Wang, R, Tischner, R, Gutiérrez, RA, Hoffman, M, Xing, X, Chen, M, Coruzzi, G & Crawford, NM 2004, 'Genomic analysis of the nitrate response using a nitrate reductase-null mutant of arabidopsis', Plant Physiology, vol. 136, no. 1, pp. 2512-2522. https://doi.org/10.1104/pp.104.044610
Wang R, Tischner R, Gutiérrez RA, Hoffman M, Xing X, Chen M et al. Genomic analysis of the nitrate response using a nitrate reductase-null mutant of arabidopsis. Plant Physiology. 2004 Sep;136(1):2512-2522. https://doi.org/10.1104/pp.104.044610
Wang, Rongchen ; Tischner, Rudolf ; Gutiérrez, Rodrigo A. ; Hoffman, Maren ; Xing, Xiujuan ; Chen, Mingsheng ; Coruzzi, Gloria ; Crawford, Nigel M. / Genomic analysis of the nitrate response using a nitrate reductase-null mutant of arabidopsis. In: Plant Physiology. 2004 ; Vol. 136, No. 1. pp. 2512-2522.
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