Genome-wide analysis of the stationary-phase sigma factor (Sigma-H) regulon of Bacillus subtilis

Robert A. Britton, Patrick Eichenberger, Jose Eduardo Gonzalez-Pastor, Paul Fawcett, Rita Monson, Richard Losick, Alan D. Grossman

Research output: Contribution to journalArticle

Abstract

Sigma-H is an alternative RNA polymerase sigma factor that directs the transcription of many genes that function at the transition from exponential growth to stationary phase in Bacillus subtilis. Twenty-three promoters, which drive transcription of 33 genes, are known to be recognized by sigma-H-containing RNA polymerase. To identify additional genes under the control of sigma-H on a genome-wide basis, we carried out transcriptional profiling experiments using a DNA microarray containing >99% of the annotated B. subtilis open reading frames. In addition, we used a bioinformatics-based approach aimed at the identification of promoters recognized by RNA polymerase containing sigma-H. This combination of approaches was successful in confirming most of the previously described sigma-H-controlled genes. In addition, we identified 26 putative promoters that drive expression of 54 genes not previously known to be under the direct control of sigma-H. Based on the known or inferred function of most of these genes, we conclude that, in addition to its previously known roles in sporulation and competence, sigma-H controls genes involved in many physiological processes associated with the transition to stationary phase, including cytochrome biogenesis, generation of potential nutrient sources, transport, and cell wall metabolism.

Original languageEnglish (US)
Pages (from-to)4881-4890
Number of pages10
JournalJournal of Bacteriology
Volume184
Issue number17
DOIs
StatePublished - Sep 2002

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Sigma Factor
Regulon
Bacillus subtilis
Genome
DNA-Directed RNA Polymerases
Genes
Physiological Phenomena
Cytochromes
Oligonucleotide Array Sequence Analysis
Computational Biology
Mental Competency
Cell Wall
Open Reading Frames
Gene Expression
Food
Growth

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Britton, R. A., Eichenberger, P., Gonzalez-Pastor, J. E., Fawcett, P., Monson, R., Losick, R., & Grossman, A. D. (2002). Genome-wide analysis of the stationary-phase sigma factor (Sigma-H) regulon of Bacillus subtilis. Journal of Bacteriology, 184(17), 4881-4890. https://doi.org/10.1128/JB.184.17.4881-4890.2002

Genome-wide analysis of the stationary-phase sigma factor (Sigma-H) regulon of Bacillus subtilis. / Britton, Robert A.; Eichenberger, Patrick; Gonzalez-Pastor, Jose Eduardo; Fawcett, Paul; Monson, Rita; Losick, Richard; Grossman, Alan D.

In: Journal of Bacteriology, Vol. 184, No. 17, 09.2002, p. 4881-4890.

Research output: Contribution to journalArticle

Britton, RA, Eichenberger, P, Gonzalez-Pastor, JE, Fawcett, P, Monson, R, Losick, R & Grossman, AD 2002, 'Genome-wide analysis of the stationary-phase sigma factor (Sigma-H) regulon of Bacillus subtilis', Journal of Bacteriology, vol. 184, no. 17, pp. 4881-4890. https://doi.org/10.1128/JB.184.17.4881-4890.2002
Britton, Robert A. ; Eichenberger, Patrick ; Gonzalez-Pastor, Jose Eduardo ; Fawcett, Paul ; Monson, Rita ; Losick, Richard ; Grossman, Alan D. / Genome-wide analysis of the stationary-phase sigma factor (Sigma-H) regulon of Bacillus subtilis. In: Journal of Bacteriology. 2002 ; Vol. 184, No. 17. pp. 4881-4890.
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