Genetic Removal of p70 S6 Kinase 1 Corrects Molecular, Synaptic, and Behavioral Phenotypes in Fragile X Syndrome Mice

Aditi Bhattacharya, Hanoch Kaphzan, Amanda C. Alvarez-Dieppa, Jaclyn P. Murphy, Philippe Pierre, Eric Klann

Research output: Contribution to journalArticle

Abstract

The ability to chronically monitor neuronal activity in the living brain is essential for understanding the organization and function of the nervous system. The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, processes, and synapses that are triggered by neuronal activities. Here we report the generation and characterization of transgenic mice that express improved GCaMPs in various neuronal subpopulations under the control of the Thy1 promoter. In vitro and in vivo studies show that calcium transients induced by spontaneous and stimulus-evoked neuronal activities can be readily detected at the level of individual cells and synapses in acute brain slices, as well as chronically in awake, behaving animals. These GCaMP transgenic mice allow investigation of activity patterns in defined neuronal populations in the living brain and will greatly facilitate dissecting complex structural and functional relationships of neural networks.

Original languageEnglish (US)
Pages (from-to)325-337
Number of pages13
JournalNeuron
Volume76
Issue number2
DOIs
StatePublished - Oct 18 2012

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70-kDa Ribosomal Protein S6 Kinases
Fragile X Syndrome
Calcium
Phenotype
Synapses
Transgenic Mice
Brain
Living Wills
Carisoprodol
Green Fluorescent Proteins
Nervous System
Organizations
Population

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Genetic Removal of p70 S6 Kinase 1 Corrects Molecular, Synaptic, and Behavioral Phenotypes in Fragile X Syndrome Mice. / Bhattacharya, Aditi; Kaphzan, Hanoch; Alvarez-Dieppa, Amanda C.; Murphy, Jaclyn P.; Pierre, Philippe; Klann, Eric.

In: Neuron, Vol. 76, No. 2, 18.10.2012, p. 325-337.

Research output: Contribution to journalArticle

Bhattacharya, Aditi ; Kaphzan, Hanoch ; Alvarez-Dieppa, Amanda C. ; Murphy, Jaclyn P. ; Pierre, Philippe ; Klann, Eric. / Genetic Removal of p70 S6 Kinase 1 Corrects Molecular, Synaptic, and Behavioral Phenotypes in Fragile X Syndrome Mice. In: Neuron. 2012 ; Vol. 76, No. 2. pp. 325-337.
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