Genetic diversity of the 2013-14 human isolates of influenza H7N9 in China

Amber Farooqui, Alberto J. Leon, Linxi Huang, Suwu Wu, Yingmu Cai, Pengzhou Lin, Weihong Chen, Xibin Fang, Tiansheng Zeng, Yisu Liu, Li Zhang, Ting Su, Weibin Chen, Elodie Ghedin, Huachen Zhu, Yi Guan, David J. Kelvin

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Influenza H7N9 has become an endemic pathogen in China where circulating virus is found extensively in wild birds and domestic poultry. Two epidemic waves of Human H7N9 infections have taken place in Eastern and South Central China during the years of 2013 and 2014. In this study, we report on the first four human cases of influenza H7N9 in Shantou, Guangdong province, which occurred during the second H7N9 wave, and the subsequent analysis of the viral isolates.

METHODS: Viral genomes were subjected to multisegment amplification and sequenced in an Illumina MiSeq. Later, phylogenetic analyses of influenza H7N9 viruses were performed to establish the evolutionary context of the disease in humans.

RESULTS: The sequences of the isolates from Shantou have closer evolutionary proximity to the predominant Eastern H7N9 cluster (similar to A/Shanghai/1/2013 (H7N9)) than to the Southern H7N9 cluster (similar to A/Guangdong/1/2013 (H7N9)).

CONCLUSIONS: Two distinct phylogenetic groups of influenza H7N9 circulate currently in China and cause infections in humans as a consequence of cross-species spillover from the avian disease. The Eastern cluster, which includes the four isolates from Shantou, presents a wide geographic distribution and overlaps with the more restricted area of circulation of the Southern cluster. Continued monitoring of the avian disease is of critical importance to better understand and predict the epidemiological behaviour of the human cases.

Original languageEnglish (US)
Pages (from-to)109
Number of pages1
JournalBMC Infectious Diseases
Volume15
DOIs
StatePublished - 2015

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Human Influenza
China
Bird Diseases
H7N9 Subtype Influenza A Virus
Viral Genome
Poultry
Infection
Orthomyxoviridae
Birds
Viruses

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Farooqui, A., Leon, A. J., Huang, L., Wu, S., Cai, Y., Lin, P., ... Kelvin, D. J. (2015). Genetic diversity of the 2013-14 human isolates of influenza H7N9 in China. BMC Infectious Diseases, 15, 109. https://doi.org/10.1186/s12879-015-0829-8

Genetic diversity of the 2013-14 human isolates of influenza H7N9 in China. / Farooqui, Amber; Leon, Alberto J.; Huang, Linxi; Wu, Suwu; Cai, Yingmu; Lin, Pengzhou; Chen, Weihong; Fang, Xibin; Zeng, Tiansheng; Liu, Yisu; Zhang, Li; Su, Ting; Chen, Weibin; Ghedin, Elodie; Zhu, Huachen; Guan, Yi; Kelvin, David J.

In: BMC Infectious Diseases, Vol. 15, 2015, p. 109.

Research output: Contribution to journalArticle

Farooqui, A, Leon, AJ, Huang, L, Wu, S, Cai, Y, Lin, P, Chen, W, Fang, X, Zeng, T, Liu, Y, Zhang, L, Su, T, Chen, W, Ghedin, E, Zhu, H, Guan, Y & Kelvin, DJ 2015, 'Genetic diversity of the 2013-14 human isolates of influenza H7N9 in China', BMC Infectious Diseases, vol. 15, pp. 109. https://doi.org/10.1186/s12879-015-0829-8
Farooqui, Amber ; Leon, Alberto J. ; Huang, Linxi ; Wu, Suwu ; Cai, Yingmu ; Lin, Pengzhou ; Chen, Weihong ; Fang, Xibin ; Zeng, Tiansheng ; Liu, Yisu ; Zhang, Li ; Su, Ting ; Chen, Weibin ; Ghedin, Elodie ; Zhu, Huachen ; Guan, Yi ; Kelvin, David J. / Genetic diversity of the 2013-14 human isolates of influenza H7N9 in China. In: BMC Infectious Diseases. 2015 ; Vol. 15. pp. 109.
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abstract = "BACKGROUND: Influenza H7N9 has become an endemic pathogen in China where circulating virus is found extensively in wild birds and domestic poultry. Two epidemic waves of Human H7N9 infections have taken place in Eastern and South Central China during the years of 2013 and 2014. In this study, we report on the first four human cases of influenza H7N9 in Shantou, Guangdong province, which occurred during the second H7N9 wave, and the subsequent analysis of the viral isolates.METHODS: Viral genomes were subjected to multisegment amplification and sequenced in an Illumina MiSeq. Later, phylogenetic analyses of influenza H7N9 viruses were performed to establish the evolutionary context of the disease in humans.RESULTS: The sequences of the isolates from Shantou have closer evolutionary proximity to the predominant Eastern H7N9 cluster (similar to A/Shanghai/1/2013 (H7N9)) than to the Southern H7N9 cluster (similar to A/Guangdong/1/2013 (H7N9)).CONCLUSIONS: Two distinct phylogenetic groups of influenza H7N9 circulate currently in China and cause infections in humans as a consequence of cross-species spillover from the avian disease. The Eastern cluster, which includes the four isolates from Shantou, presents a wide geographic distribution and overlaps with the more restricted area of circulation of the Southern cluster. Continued monitoring of the avian disease is of critical importance to better understand and predict the epidemiological behaviour of the human cases.",
author = "Amber Farooqui and Leon, {Alberto J.} and Linxi Huang and Suwu Wu and Yingmu Cai and Pengzhou Lin and Weihong Chen and Xibin Fang and Tiansheng Zeng and Yisu Liu and Li Zhang and Ting Su and Weibin Chen and Elodie Ghedin and Huachen Zhu and Yi Guan and Kelvin, {David J.}",
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T1 - Genetic diversity of the 2013-14 human isolates of influenza H7N9 in China

AU - Farooqui, Amber

AU - Leon, Alberto J.

AU - Huang, Linxi

AU - Wu, Suwu

AU - Cai, Yingmu

AU - Lin, Pengzhou

AU - Chen, Weihong

AU - Fang, Xibin

AU - Zeng, Tiansheng

AU - Liu, Yisu

AU - Zhang, Li

AU - Su, Ting

AU - Chen, Weibin

AU - Ghedin, Elodie

AU - Zhu, Huachen

AU - Guan, Yi

AU - Kelvin, David J.

PY - 2015

Y1 - 2015

N2 - BACKGROUND: Influenza H7N9 has become an endemic pathogen in China where circulating virus is found extensively in wild birds and domestic poultry. Two epidemic waves of Human H7N9 infections have taken place in Eastern and South Central China during the years of 2013 and 2014. In this study, we report on the first four human cases of influenza H7N9 in Shantou, Guangdong province, which occurred during the second H7N9 wave, and the subsequent analysis of the viral isolates.METHODS: Viral genomes were subjected to multisegment amplification and sequenced in an Illumina MiSeq. Later, phylogenetic analyses of influenza H7N9 viruses were performed to establish the evolutionary context of the disease in humans.RESULTS: The sequences of the isolates from Shantou have closer evolutionary proximity to the predominant Eastern H7N9 cluster (similar to A/Shanghai/1/2013 (H7N9)) than to the Southern H7N9 cluster (similar to A/Guangdong/1/2013 (H7N9)).CONCLUSIONS: Two distinct phylogenetic groups of influenza H7N9 circulate currently in China and cause infections in humans as a consequence of cross-species spillover from the avian disease. The Eastern cluster, which includes the four isolates from Shantou, presents a wide geographic distribution and overlaps with the more restricted area of circulation of the Southern cluster. Continued monitoring of the avian disease is of critical importance to better understand and predict the epidemiological behaviour of the human cases.

AB - BACKGROUND: Influenza H7N9 has become an endemic pathogen in China where circulating virus is found extensively in wild birds and domestic poultry. Two epidemic waves of Human H7N9 infections have taken place in Eastern and South Central China during the years of 2013 and 2014. In this study, we report on the first four human cases of influenza H7N9 in Shantou, Guangdong province, which occurred during the second H7N9 wave, and the subsequent analysis of the viral isolates.METHODS: Viral genomes were subjected to multisegment amplification and sequenced in an Illumina MiSeq. Later, phylogenetic analyses of influenza H7N9 viruses were performed to establish the evolutionary context of the disease in humans.RESULTS: The sequences of the isolates from Shantou have closer evolutionary proximity to the predominant Eastern H7N9 cluster (similar to A/Shanghai/1/2013 (H7N9)) than to the Southern H7N9 cluster (similar to A/Guangdong/1/2013 (H7N9)).CONCLUSIONS: Two distinct phylogenetic groups of influenza H7N9 circulate currently in China and cause infections in humans as a consequence of cross-species spillover from the avian disease. The Eastern cluster, which includes the four isolates from Shantou, presents a wide geographic distribution and overlaps with the more restricted area of circulation of the Southern cluster. Continued monitoring of the avian disease is of critical importance to better understand and predict the epidemiological behaviour of the human cases.

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