Genetic and genomic toolbox of the Chordate Ciona intestinalis

Alberto Stolfi, Lionel Christiaen

Research output: Contribution to journalArticle

Abstract

The experimental malleability and unique phylogenetic position of the sea squirt Ciona intestinalis as part of the sister group to the vertebrates have helped establish these marine chordates as model organisms for the study of developmental genetics and evolution. Here we summarize the tools, techniques, and resources available to the Ciona geneticist, citing examples of studies that employed such strategies in the elucidation of gene function in Ciona. Genetic screens, germline transgenesis, electroporation of plasmid DNA, and microinjection of morpholinos are all routinely employed, and in the near future we expect these to be complemented by targeted mutagenesis, homologous recombination, and RNAi. The genomic resources available will continue to support the design and interpretation of genetic experiments and allow for increasingly sophisticated approaches on a high-throughput, wholegenome scale.

Original languageEnglish (US)
Pages (from-to)55-66
Number of pages12
JournalGenetics
Volume192
Issue number1
DOIs
StatePublished - Sep 1 2012

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Ciona intestinalis
Chordata
Morpholinos
Gene Transfer Techniques
Electroporation
Molecular Evolution
Homologous Recombination
Microinjections
RNA Interference
Oceans and Seas
Mutagenesis
Vertebrates
Plasmids
DNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Genetic and genomic toolbox of the Chordate Ciona intestinalis. / Stolfi, Alberto; Christiaen, Lionel.

In: Genetics, Vol. 192, No. 1, 01.09.2012, p. 55-66.

Research output: Contribution to journalArticle

Stolfi, Alberto ; Christiaen, Lionel. / Genetic and genomic toolbox of the Chordate Ciona intestinalis. In: Genetics. 2012 ; Vol. 192, No. 1. pp. 55-66.
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