Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip

R. Machold, C. Klein, Gordon Fishell

Research output: Contribution to journalArticle

Abstract

During embryogenesis, the rhombic lip of the fourth ventricle is the germinal origin of a diverse collection of neuronal populations that ultimately reside in the brainstem and cerebellum. Rhombic lip neurogenesis requires the bHLH transcription factor Atoh1 (Math1), and commences shortly after neural tube closure (E9.5). Within the rhombomere 1 - isthmus region, the rhombic lip first produces brainstem and deep cerebellar neurons (E9.5-E12), followed by granule cell precursors after E12. While Atoh1 function is essential for all of these populations to be specified, the downstream genetic programs that confer specific properties to early and late born Atoh1 lineages are not well characterized. We have performed a comparative microarray analysis of gene expression within early and later born cohorts of Atoh1 expressing neural precursors purified from E14.5 embryos using a transgenic labeling strategy. We identify novel transcription factors, cell surface molecules, and cell cycle regulators within each pool of Atoh1 lineages that likely contribute to their distinct developmental trajectories and cell fates. In particular, our analysis reveals new insights into the genetic programs that regulate the specification and proliferation of granule cell precursors, the putative cell of origin for the majority of medulloblastomas.

Original languageEnglish (US)
Pages (from-to)349-359
Number of pages11
JournalGene Expression Patterns
Volume11
Issue number5-6
DOIs
StatePublished - Jun 1 2011

Fingerprint

Lip
Genes
Brain Stem
Basic Helix-Loop-Helix Transcription Factors
Fourth Ventricle
Medulloblastoma
Neural Tube
Neurogenesis
Microarray Analysis
Cerebellum
Population
Embryonic Development
Cell Cycle
Transcription Factors
Embryonic Structures
Cell Proliferation
Gene Expression
Neurons

Keywords

  • Atoh1
  • Brainstem
  • Cerebellum
  • Gene expression screen
  • Genetic fate mapping
  • Granule cell precursors
  • Isthmus
  • Math1
  • Microarrays
  • Neurogenesis
  • Rhombic lip
  • Rhombomere 1

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Developmental Biology

Cite this

Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip. / Machold, R.; Klein, C.; Fishell, Gordon.

In: Gene Expression Patterns, Vol. 11, No. 5-6, 01.06.2011, p. 349-359.

Research output: Contribution to journalArticle

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