Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip

R. Machold, C. Klein, Gordon Fishell

    Research output: Contribution to journalArticle

    Abstract

    During embryogenesis, the rhombic lip of the fourth ventricle is the germinal origin of a diverse collection of neuronal populations that ultimately reside in the brainstem and cerebellum. Rhombic lip neurogenesis requires the bHLH transcription factor Atoh1 (Math1), and commences shortly after neural tube closure (E9.5). Within the rhombomere 1 - isthmus region, the rhombic lip first produces brainstem and deep cerebellar neurons (E9.5-E12), followed by granule cell precursors after E12. While Atoh1 function is essential for all of these populations to be specified, the downstream genetic programs that confer specific properties to early and late born Atoh1 lineages are not well characterized. We have performed a comparative microarray analysis of gene expression within early and later born cohorts of Atoh1 expressing neural precursors purified from E14.5 embryos using a transgenic labeling strategy. We identify novel transcription factors, cell surface molecules, and cell cycle regulators within each pool of Atoh1 lineages that likely contribute to their distinct developmental trajectories and cell fates. In particular, our analysis reveals new insights into the genetic programs that regulate the specification and proliferation of granule cell precursors, the putative cell of origin for the majority of medulloblastomas.

    Original languageEnglish (US)
    Pages (from-to)349-359
    Number of pages11
    JournalGene Expression Patterns
    Volume11
    Issue number5-6
    DOIs
    StatePublished - Jun 1 2011

    Fingerprint

    Lip
    Genes
    Brain Stem
    Basic Helix-Loop-Helix Transcription Factors
    Fourth Ventricle
    Medulloblastoma
    Neural Tube
    Neurogenesis
    Microarray Analysis
    Cerebellum
    Population
    Embryonic Development
    Cell Cycle
    Transcription Factors
    Embryonic Structures
    Cell Proliferation
    Gene Expression
    Neurons

    Keywords

    • Atoh1
    • Brainstem
    • Cerebellum
    • Gene expression screen
    • Genetic fate mapping
    • Granule cell precursors
    • Isthmus
    • Math1
    • Microarrays
    • Neurogenesis
    • Rhombic lip
    • Rhombomere 1

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology
    • Developmental Biology

    Cite this

    Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip. / Machold, R.; Klein, C.; Fishell, Gordon.

    In: Gene Expression Patterns, Vol. 11, No. 5-6, 01.06.2011, p. 349-359.

    Research output: Contribution to journalArticle

    Machold, R. ; Klein, C. ; Fishell, Gordon. / Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip. In: Gene Expression Patterns. 2011 ; Vol. 11, No. 5-6. pp. 349-359.
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