Generation of long-term human cytolytic cell lines with persistent natural killer activity

A. M. Krensky, K. A. Ault, Carol Reiss, J. L. Strominger, S. J. Burakoff

Research output: Contribution to journalArticle

Abstract

Human cell lines maintained by in vitro stimulation with the HLA-A, B-negative, DR-positive, Epstein Barr virus-transformed, lymphoblastoid cell line Daudi in the presence of conditioned medium demonstrated significant NK activity for over 6 wk in continuous culture. These cells lyse K562 and a broad panel of lymphoblastoid cell lines but do not lyse normal peripheral blood lymphocytes or pokeweed mitogen blasts. They possess the sheep red blood cell receptor but lack other T cell markers (Lyt-3+, OKT3-). Natural killer activity correlated with the presence of a Mac 1-positive subpopulation of cells present in these long-term lines.

Original languageEnglish (US)
Pages (from-to)1748-1751
Number of pages4
JournalJournal of Immunology
Volume129
Issue number4
StatePublished - 1982

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Muromonab-CD3
Cell Line
Pokeweed Mitogens
Transformed Cell Line
HLA-A Antigens
HLA-B Antigens
K562 Cells
Conditioned Culture Medium
Human Herpesvirus 4
Sheep
Erythrocytes
Lymphocytes
T-Lymphocytes
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Krensky, A. M., Ault, K. A., Reiss, C., Strominger, J. L., & Burakoff, S. J. (1982). Generation of long-term human cytolytic cell lines with persistent natural killer activity. Journal of Immunology, 129(4), 1748-1751.

Generation of long-term human cytolytic cell lines with persistent natural killer activity. / Krensky, A. M.; Ault, K. A.; Reiss, Carol; Strominger, J. L.; Burakoff, S. J.

In: Journal of Immunology, Vol. 129, No. 4, 1982, p. 1748-1751.

Research output: Contribution to journalArticle

Krensky, AM, Ault, KA, Reiss, C, Strominger, JL & Burakoff, SJ 1982, 'Generation of long-term human cytolytic cell lines with persistent natural killer activity', Journal of Immunology, vol. 129, no. 4, pp. 1748-1751.
Krensky, A. M. ; Ault, K. A. ; Reiss, Carol ; Strominger, J. L. ; Burakoff, S. J. / Generation of long-term human cytolytic cell lines with persistent natural killer activity. In: Journal of Immunology. 1982 ; Vol. 129, No. 4. pp. 1748-1751.
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