Generation of inositol phosphates in bitter taste transduction

Andrew Spielman, Taufiqul Huque, Hajime Nagai, Glayde Whitney, Joseph G. Brand

Research output: Contribution to journalArticle

Abstract

It is probable that there is a diversity of mechanisms involved in the transduction of bitter taste. One of these mechanisms uses the second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). Partial membrane preparations from circumvallate and foliate taste regions of mice tongues responded to the addition of known bitter taste stimuli by increasing the amount of inositol phosphates produced after 30 s incubation. Addition of both the bitter stimulus, sucrose octaacetate and the G-protein stimulant, GTPγS, led to an enhanced production of inositol phosphates compared with either alone. Pretreatment of the tissue samples with pertussis toxin eliminated all response to sucrose octaacetate plus GTPγS, whereas pretreatment with cholera toxin was without effect. Western blots of solubilized tissue from circumvallate and foliate regions probed with antibodies to the α-subunit of several types of G-proteins revealed bands reactive to antibodies against Gαi1-2 and Gαo, with no apparent activity to antibodies against Gαi3. Given the results from the immunoblots and those of the toxin experiments, it is proposed that the transduction of the bitter taste of sucrose octaacetate in mice involves a receptor-mediated activation of a Gi-type protein which activates a phospholipase C to produce the two second messengers, IP3 and DAG.

Original languageEnglish (US)
Pages (from-to)1149-1155
Number of pages7
JournalPhysiology and Behavior
Volume56
Issue number6
DOIs
StatePublished - 1994

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Inositol Phosphates
Diglycerides
Second Messenger Systems
GTP-Binding Proteins
Antibodies
Inositol 1,4,5-Trisphosphate
Cholera Toxin
Pertussis Toxin
Type C Phospholipases
Tongue
Western Blotting
Membranes
sucrose octaacetate
Proteins

Keywords

  • Bitter
  • G-Proteins
  • Inositol 1,4,5-trisphosphate
  • Inositol phosphates
  • Phospholipase C
  • Signal transduction
  • Taste

ASJC Scopus subject areas

  • Physiology (medical)
  • Behavioral Neuroscience

Cite this

Generation of inositol phosphates in bitter taste transduction. / Spielman, Andrew; Huque, Taufiqul; Nagai, Hajime; Whitney, Glayde; Brand, Joseph G.

In: Physiology and Behavior, Vol. 56, No. 6, 1994, p. 1149-1155.

Research output: Contribution to journalArticle

Spielman, Andrew ; Huque, Taufiqul ; Nagai, Hajime ; Whitney, Glayde ; Brand, Joseph G. / Generation of inositol phosphates in bitter taste transduction. In: Physiology and Behavior. 1994 ; Vol. 56, No. 6. pp. 1149-1155.
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