Generation of guanine-thymine cross-links in human cells by one-electron oxidation mechanisms

Guru S. Madugundu, J. Richard Wagner, Jean Cadet, Konstantin Kropachev, Byeong Hwa Yun, Nicholas Geacintov, Vladimir Shafirovich

Research output: Contribution to journalArticle

Abstract

The one-electron oxidation of cellular DNA in cultured human HeLa cells initiated by intense nanosecond 266 nm laser pulse irradiation produces cross-links between guanine and thymine bases (G*-T*), characterized by a covalent bond between C8 guanine (G*) and N3 thymine (T*) atoms. The DNA lesions were quantified by isotope dilution LC-MS/MS methods in the multiple reaction-monitoring mode using isotopically labeled [ 15N, 13C]-nucleotides as internal standards. Among several known pyrimidine and 8-oxo-7,8-dihydroguanine lesions, the G*-T* cross-linked lesions were detected at levels of ∼0.21 and 1.19 d(G*-T*) lesions per 106 DNA bases at laser intensities of 50 and 280 mJ/cm2/pulse, respectively.

Original languageEnglish (US)
Pages (from-to)1031-1033
Number of pages3
JournalChemical Research in Toxicology
Volume26
Issue number7
DOIs
StatePublished - Jul 15 2013

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Thymine
Guanine
Cells
Electrons
Oxidation
Laser pulses
DNA
Lasers
Covalent bonds
HeLa Cells
Isotopes
Dilution
Nucleotides
Irradiation
Atoms
Monitoring

ASJC Scopus subject areas

  • Toxicology
  • Medicine(all)

Cite this

Madugundu, G. S., Wagner, J. R., Cadet, J., Kropachev, K., Yun, B. H., Geacintov, N., & Shafirovich, V. (2013). Generation of guanine-thymine cross-links in human cells by one-electron oxidation mechanisms. Chemical Research in Toxicology, 26(7), 1031-1033. https://doi.org/10.1021/tx400158g

Generation of guanine-thymine cross-links in human cells by one-electron oxidation mechanisms. / Madugundu, Guru S.; Wagner, J. Richard; Cadet, Jean; Kropachev, Konstantin; Yun, Byeong Hwa; Geacintov, Nicholas; Shafirovich, Vladimir.

In: Chemical Research in Toxicology, Vol. 26, No. 7, 15.07.2013, p. 1031-1033.

Research output: Contribution to journalArticle

Madugundu, GS, Wagner, JR, Cadet, J, Kropachev, K, Yun, BH, Geacintov, N & Shafirovich, V 2013, 'Generation of guanine-thymine cross-links in human cells by one-electron oxidation mechanisms', Chemical Research in Toxicology, vol. 26, no. 7, pp. 1031-1033. https://doi.org/10.1021/tx400158g
Madugundu, Guru S. ; Wagner, J. Richard ; Cadet, Jean ; Kropachev, Konstantin ; Yun, Byeong Hwa ; Geacintov, Nicholas ; Shafirovich, Vladimir. / Generation of guanine-thymine cross-links in human cells by one-electron oxidation mechanisms. In: Chemical Research in Toxicology. 2013 ; Vol. 26, No. 7. pp. 1031-1033.
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