Generation of a radial-like glial cell line

David R. Friedlander, Perry A. Brittis, Takeshi Sakurai, Bronya Shif, William Wirchansky, Gordon Fishell, Martin Grumet

    Research output: Contribution to journalArticle

    Abstract

    Rat C6 glioma is a cell line that has been used extensively as a model of astroglia. Although this cell line retains many of the properties of developing glia, it does not resemble morphologically the specialized form of glia found embryonically, the radial glia. In experiments designed to study a mutant form of receptor protein tyrosine phosphatase β, we isolated a subclone of C6 called C6-R which, like radial glia, assumes a highly polarized radial-like morphology in culture. C6-R cells and, to a somewhat lesser extent, C6 cells, express cytoskeletal proteins found in developing astroglia including glial fibrillary acidic protein and RC1. As seen with radial glia, cerebellar granule cell bodies and neurites migrated along radial processes of C6-R cells in culture. Morphological analysis of dye- labeled cells injected into the developing forebrain revealed that a large fraction (~60%) of the C6-R cells in the cortex assumed a radial orientation and about half of these (~30%) made contact with the pial surface. In contrast, the parental C6 cells generally formed aggregates and only displayed a radial alignment when associated with blood vessels. These results suggest that we have generated a stable cell line from C6 glioma which has adopted certain key features of radial glia, including the ability to promote neuronal migration in culture and integrate radially in vivo in response to local cues. This cell line may be particularly useful for studying receptors on radial glia that mediate neuronal migration.

    Original languageEnglish (US)
    Pages (from-to)291-304
    Number of pages14
    JournalJournal of Neurobiology
    Volume37
    Issue number2
    DOIs
    StatePublished - Nov 5 1998

    Fingerprint

    Ependymoglial Cells
    Neuroglia
    Cell Line
    Glioma
    Astrocytes
    Protein Tyrosine Phosphatases
    Aptitude
    Cytoskeletal Proteins
    Glial Fibrillary Acidic Protein
    Neurites
    Prosencephalon
    Cues
    Blood Vessels
    Coloring Agents
    Cell Culture Techniques

    Keywords

    • C6 glioma
    • Cerebellar granule cells
    • Neuronal migration
    • Radial glia
    • RPTPβ

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Friedlander, D. R., Brittis, P. A., Sakurai, T., Shif, B., Wirchansky, W., Fishell, G., & Grumet, M. (1998). Generation of a radial-like glial cell line. Journal of Neurobiology, 37(2), 291-304. https://doi.org/10.1002/(SICI)1097-4695(19981105)37:2<291::AID-NEU8>3.0.CO;2-F

    Generation of a radial-like glial cell line. / Friedlander, David R.; Brittis, Perry A.; Sakurai, Takeshi; Shif, Bronya; Wirchansky, William; Fishell, Gordon; Grumet, Martin.

    In: Journal of Neurobiology, Vol. 37, No. 2, 05.11.1998, p. 291-304.

    Research output: Contribution to journalArticle

    Friedlander, DR, Brittis, PA, Sakurai, T, Shif, B, Wirchansky, W, Fishell, G & Grumet, M 1998, 'Generation of a radial-like glial cell line', Journal of Neurobiology, vol. 37, no. 2, pp. 291-304. https://doi.org/10.1002/(SICI)1097-4695(19981105)37:2<291::AID-NEU8>3.0.CO;2-F
    Friedlander DR, Brittis PA, Sakurai T, Shif B, Wirchansky W, Fishell G et al. Generation of a radial-like glial cell line. Journal of Neurobiology. 1998 Nov 5;37(2):291-304. https://doi.org/10.1002/(SICI)1097-4695(19981105)37:2<291::AID-NEU8>3.0.CO;2-F
    Friedlander, David R. ; Brittis, Perry A. ; Sakurai, Takeshi ; Shif, Bronya ; Wirchansky, William ; Fishell, Gordon ; Grumet, Martin. / Generation of a radial-like glial cell line. In: Journal of Neurobiology. 1998 ; Vol. 37, No. 2. pp. 291-304.
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