Gene synteny and chloroquine resistance in Plasmodium chabaudi

Paul Hunt, Axel Martinelli, Richard Fawcett, Jane Carlton, Richard Carter, David Walliker

Research output: Contribution to journalArticle

Abstract

Chloroquine resistance in the rodent malaria parasite Plasmodium chabaudi has been shown to be caused by a gene on chromosome 11, and is not linked to orthologues of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) or Pgh-1 (pfmdr1) genes. In the current work, the progeny of crosses between chloroquine-resistant and sensitive clones of P. chabaudi have been analysed for the inheritance of 658 AFLP markers. Markers linked to the chloroquine responses of the progeny, including two which are completely linked, have been genetically mapped, sequenced and their homologues, or closely linked loci, identified in P. falciparum. The chromosome 11 markers most closely linked to chloroquine resistance in P. chabaudi map to loci which are also closely linked in P. falciparum, although in two linkage groups on chromosomes 6 and 13 of this species. The P. falciparum orthologue of the gene conferring chloroquine resistance in P. chabaudi is predicted to lie within a 250 kb region of P. falciparum chromosome 6, containing approximately 50 genes. The genetic order of the markers in P. chabaudi is co-linear with the physical linkage represented in the P. falciparum genome database. The findings provide evidence for extensive conservation of synteny between the two species.

Original languageEnglish (US)
Pages (from-to)157-164
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume136
Issue number2
DOIs
StatePublished - Aug 2004

Fingerprint

Plasmodium chabaudi
Synteny
Chloroquine
Plasmodium falciparum
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 6
Genes
Genetic Markers
Chromosomes, Human, Pair 13
Malaria
Rodentia
Parasites
Clone Cells
Genome
Databases

Keywords

  • AFLP
  • amplified fragment length polymorphism
  • deoxyribonucleic acid
  • DNA
  • EDTA
  • ethylenediamine tetra acetic acid
  • PBS
  • PCR
  • phosphate buffered saline
  • polymerase chain reaction
  • restriction fragment length polymorphism
  • RFLP
  • SDS
  • sodium dodecyl sulphate

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Gene synteny and chloroquine resistance in Plasmodium chabaudi. / Hunt, Paul; Martinelli, Axel; Fawcett, Richard; Carlton, Jane; Carter, Richard; Walliker, David.

In: Molecular and Biochemical Parasitology, Vol. 136, No. 2, 08.2004, p. 157-164.

Research output: Contribution to journalArticle

Hunt, Paul ; Martinelli, Axel ; Fawcett, Richard ; Carlton, Jane ; Carter, Richard ; Walliker, David. / Gene synteny and chloroquine resistance in Plasmodium chabaudi. In: Molecular and Biochemical Parasitology. 2004 ; Vol. 136, No. 2. pp. 157-164.
@article{4f94d3e00f1249809b313c4584835ac6,
title = "Gene synteny and chloroquine resistance in Plasmodium chabaudi",
abstract = "Chloroquine resistance in the rodent malaria parasite Plasmodium chabaudi has been shown to be caused by a gene on chromosome 11, and is not linked to orthologues of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) or Pgh-1 (pfmdr1) genes. In the current work, the progeny of crosses between chloroquine-resistant and sensitive clones of P. chabaudi have been analysed for the inheritance of 658 AFLP markers. Markers linked to the chloroquine responses of the progeny, including two which are completely linked, have been genetically mapped, sequenced and their homologues, or closely linked loci, identified in P. falciparum. The chromosome 11 markers most closely linked to chloroquine resistance in P. chabaudi map to loci which are also closely linked in P. falciparum, although in two linkage groups on chromosomes 6 and 13 of this species. The P. falciparum orthologue of the gene conferring chloroquine resistance in P. chabaudi is predicted to lie within a 250 kb region of P. falciparum chromosome 6, containing approximately 50 genes. The genetic order of the markers in P. chabaudi is co-linear with the physical linkage represented in the P. falciparum genome database. The findings provide evidence for extensive conservation of synteny between the two species.",
keywords = "AFLP, amplified fragment length polymorphism, deoxyribonucleic acid, DNA, EDTA, ethylenediamine tetra acetic acid, PBS, PCR, phosphate buffered saline, polymerase chain reaction, restriction fragment length polymorphism, RFLP, SDS, sodium dodecyl sulphate",
author = "Paul Hunt and Axel Martinelli and Richard Fawcett and Jane Carlton and Richard Carter and David Walliker",
year = "2004",
month = "8",
doi = "10.1016/j.molbiopara.2004.03.008",
language = "English (US)",
volume = "136",
pages = "157--164",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Gene synteny and chloroquine resistance in Plasmodium chabaudi

AU - Hunt, Paul

AU - Martinelli, Axel

AU - Fawcett, Richard

AU - Carlton, Jane

AU - Carter, Richard

AU - Walliker, David

PY - 2004/8

Y1 - 2004/8

N2 - Chloroquine resistance in the rodent malaria parasite Plasmodium chabaudi has been shown to be caused by a gene on chromosome 11, and is not linked to orthologues of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) or Pgh-1 (pfmdr1) genes. In the current work, the progeny of crosses between chloroquine-resistant and sensitive clones of P. chabaudi have been analysed for the inheritance of 658 AFLP markers. Markers linked to the chloroquine responses of the progeny, including two which are completely linked, have been genetically mapped, sequenced and their homologues, or closely linked loci, identified in P. falciparum. The chromosome 11 markers most closely linked to chloroquine resistance in P. chabaudi map to loci which are also closely linked in P. falciparum, although in two linkage groups on chromosomes 6 and 13 of this species. The P. falciparum orthologue of the gene conferring chloroquine resistance in P. chabaudi is predicted to lie within a 250 kb region of P. falciparum chromosome 6, containing approximately 50 genes. The genetic order of the markers in P. chabaudi is co-linear with the physical linkage represented in the P. falciparum genome database. The findings provide evidence for extensive conservation of synteny between the two species.

AB - Chloroquine resistance in the rodent malaria parasite Plasmodium chabaudi has been shown to be caused by a gene on chromosome 11, and is not linked to orthologues of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) or Pgh-1 (pfmdr1) genes. In the current work, the progeny of crosses between chloroquine-resistant and sensitive clones of P. chabaudi have been analysed for the inheritance of 658 AFLP markers. Markers linked to the chloroquine responses of the progeny, including two which are completely linked, have been genetically mapped, sequenced and their homologues, or closely linked loci, identified in P. falciparum. The chromosome 11 markers most closely linked to chloroquine resistance in P. chabaudi map to loci which are also closely linked in P. falciparum, although in two linkage groups on chromosomes 6 and 13 of this species. The P. falciparum orthologue of the gene conferring chloroquine resistance in P. chabaudi is predicted to lie within a 250 kb region of P. falciparum chromosome 6, containing approximately 50 genes. The genetic order of the markers in P. chabaudi is co-linear with the physical linkage represented in the P. falciparum genome database. The findings provide evidence for extensive conservation of synteny between the two species.

KW - AFLP

KW - amplified fragment length polymorphism

KW - deoxyribonucleic acid

KW - DNA

KW - EDTA

KW - ethylenediamine tetra acetic acid

KW - PBS

KW - PCR

KW - phosphate buffered saline

KW - polymerase chain reaction

KW - restriction fragment length polymorphism

KW - RFLP

KW - SDS

KW - sodium dodecyl sulphate

UR - http://www.scopus.com/inward/record.url?scp=2942525695&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942525695&partnerID=8YFLogxK

U2 - 10.1016/j.molbiopara.2004.03.008

DO - 10.1016/j.molbiopara.2004.03.008

M3 - Article

VL - 136

SP - 157

EP - 164

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 2

ER -