Gene flow in great bustard populations across the Strait of Gibraltar as elucidated from excremental PCR and mtDNA sequencing

D. Broderick, Youssef Idaghdhour, A. Korrida, J. Hellmich

Research output: Contribution to journalArticle

Abstract

Recent advances in molecular biology have made it possible to use the trace amounts of DNA in faeces to non-invasively sample endangered species for genetic studies. Here we use faeces as a source of DNA and mtDNA sequence data to elucidate the relationship among Spanish and Moroccan populations of great bustards. 834 bp of combined control region and cytochrome-b mtDNA fragments revealed four variable sites that defined seven closely related haplotypes in 54 individuals. Morocco was fixed for a single mtDNA haplotype that occurs at moderate frequency (28%) in Spain. We could not differentiate among the sampled Spanish populations of Cáceres and Andalucía but these combined populations were differentiated from the Moroccan population. Estimates of gene flow (Nm = 0.82) are consistent with extensive observations on the southern Iberian peninsular indicating that few individuals fly across the Strait of Gibraltar. We demonstrate that both this sea barrier and mountain barriers in Spain limit dispersal among adjacent great bustard populations to a similar extent. The Moroccan population is of high ornithological significance as it holds the only population of great bustards in Africa. This population is critically small and genetic and observational data indicate that it is unlikely to be recolonised via immigration from Spain should it be extirpated. In light of the evidence presented here it deserves the maximum level of protection.

Original languageEnglish (US)
Pages (from-to)793-800
Number of pages8
JournalConservation Genetics
Volume4
Issue number6
DOIs
StatePublished - Dec 1 2003

Fingerprint

Gibraltar
Gene Flow
Mitochondrial DNA
feces
gene flow
strait
mitochondrial DNA
DNA
Polymerase Chain Reaction
endangered species
immigration
Population
cytochrome
Spain
mountain
Feces
Haplotypes
haplotypes
Endangered Species
Morocco

Keywords

  • Faeces
  • Gene flow
  • mtDNA
  • Non-invasive genetics
  • Otis tarda

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Gene flow in great bustard populations across the Strait of Gibraltar as elucidated from excremental PCR and mtDNA sequencing. / Broderick, D.; Idaghdhour, Youssef; Korrida, A.; Hellmich, J.

In: Conservation Genetics, Vol. 4, No. 6, 01.12.2003, p. 793-800.

Research output: Contribution to journalArticle

@article{fb4a871dd6ed483494c4f7871a15417d,
title = "Gene flow in great bustard populations across the Strait of Gibraltar as elucidated from excremental PCR and mtDNA sequencing",
abstract = "Recent advances in molecular biology have made it possible to use the trace amounts of DNA in faeces to non-invasively sample endangered species for genetic studies. Here we use faeces as a source of DNA and mtDNA sequence data to elucidate the relationship among Spanish and Moroccan populations of great bustards. 834 bp of combined control region and cytochrome-b mtDNA fragments revealed four variable sites that defined seven closely related haplotypes in 54 individuals. Morocco was fixed for a single mtDNA haplotype that occurs at moderate frequency (28{\%}) in Spain. We could not differentiate among the sampled Spanish populations of C{\'a}ceres and Andaluc{\'i}a but these combined populations were differentiated from the Moroccan population. Estimates of gene flow (Nm = 0.82) are consistent with extensive observations on the southern Iberian peninsular indicating that few individuals fly across the Strait of Gibraltar. We demonstrate that both this sea barrier and mountain barriers in Spain limit dispersal among adjacent great bustard populations to a similar extent. The Moroccan population is of high ornithological significance as it holds the only population of great bustards in Africa. This population is critically small and genetic and observational data indicate that it is unlikely to be recolonised via immigration from Spain should it be extirpated. In light of the evidence presented here it deserves the maximum level of protection.",
keywords = "Faeces, Gene flow, mtDNA, Non-invasive genetics, Otis tarda",
author = "D. Broderick and Youssef Idaghdhour and A. Korrida and J. Hellmich",
year = "2003",
month = "12",
day = "1",
doi = "10.1023/B:COGE.0000006111.65204.c9",
language = "English (US)",
volume = "4",
pages = "793--800",
journal = "Conservation Genetics",
issn = "1566-0621",
publisher = "Springer Netherlands",
number = "6",

}

TY - JOUR

T1 - Gene flow in great bustard populations across the Strait of Gibraltar as elucidated from excremental PCR and mtDNA sequencing

AU - Broderick, D.

AU - Idaghdhour, Youssef

AU - Korrida, A.

AU - Hellmich, J.

PY - 2003/12/1

Y1 - 2003/12/1

N2 - Recent advances in molecular biology have made it possible to use the trace amounts of DNA in faeces to non-invasively sample endangered species for genetic studies. Here we use faeces as a source of DNA and mtDNA sequence data to elucidate the relationship among Spanish and Moroccan populations of great bustards. 834 bp of combined control region and cytochrome-b mtDNA fragments revealed four variable sites that defined seven closely related haplotypes in 54 individuals. Morocco was fixed for a single mtDNA haplotype that occurs at moderate frequency (28%) in Spain. We could not differentiate among the sampled Spanish populations of Cáceres and Andalucía but these combined populations were differentiated from the Moroccan population. Estimates of gene flow (Nm = 0.82) are consistent with extensive observations on the southern Iberian peninsular indicating that few individuals fly across the Strait of Gibraltar. We demonstrate that both this sea barrier and mountain barriers in Spain limit dispersal among adjacent great bustard populations to a similar extent. The Moroccan population is of high ornithological significance as it holds the only population of great bustards in Africa. This population is critically small and genetic and observational data indicate that it is unlikely to be recolonised via immigration from Spain should it be extirpated. In light of the evidence presented here it deserves the maximum level of protection.

AB - Recent advances in molecular biology have made it possible to use the trace amounts of DNA in faeces to non-invasively sample endangered species for genetic studies. Here we use faeces as a source of DNA and mtDNA sequence data to elucidate the relationship among Spanish and Moroccan populations of great bustards. 834 bp of combined control region and cytochrome-b mtDNA fragments revealed four variable sites that defined seven closely related haplotypes in 54 individuals. Morocco was fixed for a single mtDNA haplotype that occurs at moderate frequency (28%) in Spain. We could not differentiate among the sampled Spanish populations of Cáceres and Andalucía but these combined populations were differentiated from the Moroccan population. Estimates of gene flow (Nm = 0.82) are consistent with extensive observations on the southern Iberian peninsular indicating that few individuals fly across the Strait of Gibraltar. We demonstrate that both this sea barrier and mountain barriers in Spain limit dispersal among adjacent great bustard populations to a similar extent. The Moroccan population is of high ornithological significance as it holds the only population of great bustards in Africa. This population is critically small and genetic and observational data indicate that it is unlikely to be recolonised via immigration from Spain should it be extirpated. In light of the evidence presented here it deserves the maximum level of protection.

KW - Faeces

KW - Gene flow

KW - mtDNA

KW - Non-invasive genetics

KW - Otis tarda

UR - http://www.scopus.com/inward/record.url?scp=1642444048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642444048&partnerID=8YFLogxK

U2 - 10.1023/B:COGE.0000006111.65204.c9

DO - 10.1023/B:COGE.0000006111.65204.c9

M3 - Article

AN - SCOPUS:1642444048

VL - 4

SP - 793

EP - 800

JO - Conservation Genetics

JF - Conservation Genetics

SN - 1566-0621

IS - 6

ER -