Gene expression analysis with an integrated CMOS microarray by time-resolved fluorescence detection

Ta chien D. Huang, Sunirmal Paul, Ping Gong, Rastislav Levicky, John Kymissis, Sally A. Amundson, Kenneth L. Shepard

Research output: Contribution to journalArticle

Abstract

DNA microarrays have proven extraordinarily powerful for differential expression studies across thousands of genes in a single experiment. Microarrays also have the potential for clinical applications, including the detection of infectious and immunological diseases and cancer, if they can be rendered both reliable and cost-effective. Here we report the first practical application of an active microarray based on integrated circuit technology, completely obviating the need for external measurement instrumentation while employing protocols compatible with traditional fluorescence-based surface bioassays. In a gene expression biodosimetry study, we determine the differential activity of genes from leucocytes in irradiated human blood. Quantum dots are used as fluorescence labels to realize filterless, time-gated fluorescence detection on an active complementary metal-oxide-semiconductor (CMOS) microarray with 100-pM sensitivity. Improvements in surface chemistry should allow sensitivities that approach the microarray hardware limit of less than 10. pM. Techniques for covalent attachment of DNA capture strands to the CMOS active microarrays allow integrated sensors to be placed in immediate proximity to hybridized analyte strands, maximizing photon collection efficiencies.

Original languageEnglish (US)
Pages (from-to)2660-2665
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number5
DOIs
StatePublished - Jan 15 2011

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Keywords

  • CMOS biosensor
  • Integrated DNA microarray
  • Time-resolved fluorescence detection

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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