Gene-expression analysis of early- and late-maturation-stage rat enamel organ

Rodrigo Lacruz, Charles E. Smith, Yi Bu Chen, Michael J. Hubbard, Joseph G. Hacia, Michael L. Paine

Research output: Contribution to journalArticle

Abstract

Enamel maturation is a dynamic process that involves high rates of mineral acquisition, associated fluctuations in extracellular pH, and resorption of extracellular enamel proteins. During maturation, ameloblasts change from having a tall, thin, and highly polarized organization, characteristic of the secretory stage, to having a low columnar and widened morphology in the maturation stage. To identify potential differences in gene expression throughout maturation, we obtained enamel organ epithelial cells derived from the early- and late-maturation stages of rat incisor and analyzed the global gene-expression profiles at each stage. Sixty-three candidate genes were identified as having potential roles in the maturation process. Quantitative PCR was used to confirm the results of this genome-wide analysis in a subset of genes. Transcripts enriched during late maturation (n=38) included those associated with lysosomal activity, solute carrier transport, and calcium signaling. Also up-regulated were transcripts involved in cellular responses to oxidative stress, proton transport, cell death, and the immune system. Transcripts down-regulated during the late maturation stage (n=25) included those with functions related to cell adhesion, cell signaling, and T-cell activation. These results indicate that ameloblasts undergo widespread molecular changes during the maturation stage of amelogenesis and hence provide a basis for future functional investigations into the mechanistic basis of enamel mineralization.

Original languageEnglish (US)
Pages (from-to)149-157
Number of pages9
JournalEuropean Journal of Oral Sciences
Volume119
Issue numberSUPPL.1
DOIs
StatePublished - Dec 2011

Fingerprint

Enamel Organ
Dental Enamel
Ameloblasts
Gene Expression
Amelogenesis
Calcium Signaling
Incisor
Transcriptome
Cell Adhesion
Genes
Minerals
Protons
Immune System
Oxidative Stress
Cell Death
Epithelial Cells
Genome
T-Lymphocytes
Polymerase Chain Reaction
Proteins

Keywords

  • Amelogenesis
  • Early maturation
  • Enamel organ
  • Gene expression
  • Late maturation

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Gene-expression analysis of early- and late-maturation-stage rat enamel organ. / Lacruz, Rodrigo; Smith, Charles E.; Chen, Yi Bu; Hubbard, Michael J.; Hacia, Joseph G.; Paine, Michael L.

In: European Journal of Oral Sciences, Vol. 119, No. SUPPL.1, 12.2011, p. 149-157.

Research output: Contribution to journalArticle

Lacruz, Rodrigo ; Smith, Charles E. ; Chen, Yi Bu ; Hubbard, Michael J. ; Hacia, Joseph G. ; Paine, Michael L. / Gene-expression analysis of early- and late-maturation-stage rat enamel organ. In: European Journal of Oral Sciences. 2011 ; Vol. 119, No. SUPPL.1. pp. 149-157.
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